Alfonso-García Alba, Mittal Richa, Lee Eun Seong, Potma Eric O
University of California, Beckman Laser Institute, Irvine, California 92697.
Center for Nano-Bio Technology, Division of Convergence Technology, Korea Research Institute of Standards and Science, 1 Doryong-Dong, Yuseong-Gu, Daejeon 305-340, Republic of Korea.
J Biomed Opt. 2014 Jul;19(7):71407. doi: 10.1117/1.JBO.19.7.071407.
Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides information about the applicability of this nonlinear optical imaging approach for biological research.
相干拉曼散射（CRS）显微镜作为生物研究成像工具集的一项重要补充正逐渐得到认可。充分利用这种无标记成像技术，需要对CRS显微镜的物理原理和技术优点有基本的了解。本教程对CRS显微镜背后的原理进行了定性解释，并提供了有关这种非线性光学成像方法在生物研究中的适用性的信息。
