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肠易激综合征患者血小板中 5-羟色胺转运体活性和 5-羟色胺浓度:性别影响。

Serotonin transporter activity and serotonin concentration in platelets of patients with irritable bowel syndrome: effect of gender.

机构信息

Department of Psychiatry and Psychotherapy, Charité-Universitätsmedizin Berlin, Campus Charité Mitte (CCM), Schumannstr. 20-21, 10117 Berlin, Germany.

出版信息

J Gastroenterol. 2010 Apr;45(4):389-98. doi: 10.1007/s00535-009-0167-y. Epub 2009 Dec 8.

Abstract

BACKGROUND

In the past decade, a strong argument has been built for the role of serotonin (5HT) and the serotonin transporter (SERT) in irritable bowel syndrome (IBS). However, it is still not clear how SERT contributes to this clinically heterogeneous disease. The present study addressed this issue by implementing platelet (plt) markers of SERT activity in the assessment protocol.

METHODS

Fasting blood samples of 149 (51 male/98 female) subjects with Rome II and III defined IBS subtypes, and 163 healthy control subjects (CSs; 75 male/88 female) were analyzed for platelet 5HT concentration and 5HT uptake activity [maximum uptake rate (V (max)) and affinity constant (K (m))].

RESULTS

Gender had a significant impact on platelet markers of SERT activity. Male IBS patients showed significantly lower median V (max) and K (m) values than the male CS (V (max) 1.706 vs. 2.148 nmol/10(9) plts x min, P < 0.001; K (m) 346 vs. 410 nmol, P = 0.008) without any significant reduction in platelet 5HT concentration (362 vs. 394 ng/10(9) plts). On the other hand, V (max) values were not different between female IBS patients and female CS (1.642 vs. 1.741 nmol/10(9) plts x min), but platelet 5HT concentration was significantly lower in females with diarrhea-predominant IBS (363 vs. 435 ng/10(9) plts, P < 0.05).

CONCLUSION

Although an absolute extrapolation from platelets to the gastrointestinal tissue does not appear to be justified, our findings demonstrated that the contribution of disturbed SERT activity to IBS is not uniform and is possibly gender-specific. The results suggest that an assessment of SERT function in platelets may help to elucidate the differences between IBS patients in response to drugs affecting the 5HT system.

摘要

背景

在过去的十年中,强烈的观点认为 5-羟色胺(5HT)和 5-羟色胺转运体(SERT)在肠易激综合征(IBS)中发挥作用。然而,SERT 如何导致这种临床表现异质性的疾病尚不清楚。本研究通过实施血小板(plt)SERT 活性标记物来评估该问题。

方法

对 149 名(51 名男性/98 名女性)罗马 II 期和 III 期定义的 IBS 亚型患者和 163 名健康对照者(CS;75 名男性/88 名女性)的空腹血样进行了分析,以测定血小板 5HT 浓度和 5HT 摄取活性[最大摄取率(V(max))和亲和力常数(K(m))]。

结果

性别对 SERT 活性的血小板标记物有显著影响。与男性 CS 相比,男性 IBS 患者的 V(max)和 K(m)值显著降低(V(max)1.706 对 2.148 nmol/10(9)plt×min,P<0.001;K(m)346 对 410 nmol,P=0.008),但血小板 5HT 浓度无明显降低(362 对 394 ng/10(9)plt)。另一方面,女性 IBS 患者与女性 CS 之间的 V(max)值无差异(1.642 对 1.741 nmol/10(9)plt×min),但腹泻为主的 IBS 女性的血小板 5HT 浓度明显降低(363 对 435 ng/10(9)plt,P<0.05)。

结论

尽管从血小板推断到胃肠道组织似乎没有得到绝对的支持,但我们的研究结果表明,SERT 活性紊乱对 IBS 的影响并非一致,且可能具有性别特异性。结果表明,评估血小板中的 SERT 功能可能有助于阐明 5HT 系统药物治疗对 IBS 患者的影响差异。

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