Department of Clinical Biology, Division of Biochemistry, University Hospital Haukeland, University of Bergen, N-5021, Bergen, Norway.
Platelets. 1996;7(1-2):47-52. doi: 10.3109/09537109609079509.
The endogenous content of serotonin in human platelets has been used in many clinical studies to indicate platelet activation. A decrease in platelet serotonin compared with controls has been regarded as an indicator of platelet activation. However, the results published are difficult to compare, because of huge variations in endogenous serotonin between control groups in different investigations. This is likely to be because of lack of standardization. Several factors that influence the endogenous platelet serotonin content were studied in more than 200 blood donors. The most important factor was the total g force of the centrifugation used to isolate platelets. Also the age and sex of platelet donors, number of platelets in platelet-rich plasma, and mean platelet volume influenced normal serotonin values. Using a standardized centrifugation procedure (2700 g min) the mean endogenous serotonin was 2.80 nmol/10(9) platelets in young women and 2.58 in elderly women, and 2.67 in young men and 2.30 in elderly men. The differences both for age and sex were statistically significant. Endogenous platelet serotonin shows intrapersonal stability over time, since endogenous platelet serotonin did not change on repeated venepuncture for 9 weeks. Factors such as age, sex and isolation procedure must therefore be considered when endogenous platelet serotonin are studied in relation to disease and treatment.
人体内血小板中的 5-羟色胺含量在许多临床研究中被用于指示血小板的激活。与对照组相比，血小板 5-羟色胺含量降低被认为是血小板激活的指标。然而，由于不同研究中对照组之间内源性 5-羟色胺含量存在巨大差异，发表的结果难以进行比较。这可能是由于缺乏标准化。我们对 200 多名献血者进行了多项影响内源性血小板 5-羟色胺含量的因素研究。最重要的因素是分离血小板时使用的离心总重力。血小板供体的年龄和性别、富含血小板的血浆中的血小板数量以及平均血小板体积也会影响正常的 5-羟色胺值。使用标准化的离心程序（2700g/min），年轻女性的内源性 5-羟色胺平均为 2.80 nmol/10(9)血小板，老年女性为 2.58 nmol/10(9)血小板，年轻男性为 2.67 nmol/10(9)血小板，老年男性为 2.30 nmol/10(9)血小板。年龄和性别差异均具有统计学意义。由于在 9 周的时间内重复静脉穿刺时内源性血小板 5-羟色胺没有变化，因此内源性血小板 5-羟色胺随时间表现出个体内稳定性。因此，在研究与疾病和治疗相关的内源性血小板 5-羟色胺时，必须考虑年龄、性别和分离程序等因素。
