Aurello Paolo, Bellagamba Riccardo, Rossi Del Monte Simone, D'Angelo Francesco, Nigri Giuseppe, Cicchini Claudia, Ravaioli Matteo, Ramacciato Giovanni
University of Rome La Sapienza Second School of Medicine, Sant' Andrea Hospital, General Surgery Ward 3, Rome, Italy.
Am Surg. 2009 Dec;75(12):1183-8.
Gastric cancer remains one of the most common human malignancies with a poor prognosis. Apoptosis is known to be a programmed cell death and its inhibition is involved in the unregulated cellular growth that leads to neoplasms. Microvessel density (MVD) has been investigated as a promoting factor for angiogenesis with conflicting results about its relation to survival. The aim of our study was to search a correlation between these factors and some clinicopathological features and prognosis. Identification of apoptotic cells was performed applying the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique and recorded as apoptotic index (A.I.), whereas monoclonal antibodies were used for the study of MVD. A significant correlation was found between low and high A.I. and the subgroup of patients in Stages I and II (P < 0.02); 20 per cent of patients with a low A.I. showed an overall survival longer than 5 years versus 44 per cent of patients with an high A.I. (P = 0.041). High MVD was significantly related to the T stage (P = 0.036) and to a poorer 5-year overall survival (P < 0.05). Further studies are required to confirm the role of apoptosis and MVD in the development and progression of gastric cancer.
胃癌仍然是最常见的人类恶性肿瘤之一，预后较差。已知细胞凋亡是一种程序性细胞死亡，其抑制作用与导致肿瘤的细胞生长失控有关。微血管密度（MVD）已被作为血管生成的促进因素进行研究，但其与生存率的关系结果存在争议。我们研究的目的是寻找这些因素与一些临床病理特征及预后之间的相关性。应用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记技术鉴定凋亡细胞，并记录为凋亡指数（A.I.），而使用单克隆抗体研究MVD。发现低和高A.I.与Ⅰ期和Ⅱ期患者亚组之间存在显著相关性（P < 0.02）；A.I.低的患者中有20%的总生存期超过5年，而A.I.高的患者为44%（P = 0.041）。高MVD与T分期显著相关（P = 0.036），且与较差的5年总生存期相关（P < 0.05）。需要进一步研究来证实细胞凋亡和MVD在胃癌发生和发展中的作用。
