[氯胺酮诱导小鼠精神分裂症样症状与NRG1、ErbB4 mRNA表达的相关性]

[The correlation between ketamine-induced schizophrenia-like signs in mice and the expressions of NRG1, ErbB4 mRNA].

作者信息

Bian Shi-Zhong, Liu Wei-Li, Zhang Zhi-Xiang, Gu Zhen-Lun, Jiang Xiao-Gang, Guo Ci-Yi

机构信息

Department of Forensic Medicine, Medical College of Soochow University, Suzhou, China.

出版信息

Fa Yi Xue Za Zhi. 2009 Oct;25(5):348-51, 358.

PMID:20000043

Abstract

OBJECTIVE

To explore the correlation between signs similar to schizophrenia in mice after ketamine administration and the expressions of NRG1 and ErbB4 mRNA in order to explain the possible pathogenesis of schizophrenia.

METHODS

Fifty KM mice were randomly divided into 5 groups which were administered intraperitoneally with saline, clozapine and different dosages ketamine. The ketamine groups were administered intraperitoneally with low dosage (25 mg/kg), middle dosage (50 mg/kg) and high dosage (100 mg/kg) one time every day for 7 days. After administration of 100 mg/kg ketamine for 7 days, the clozapine group was introgastrically administered 20 mg/kg with clozapine one time every day for 7 days. The pathological changes of hippocampus neurons were observed by HE stain. The expressions of the NRG1 and ErbB4 mRNA in hippocampus were detected by reverse transcriptase polymerase chain reaction (RT-PCR).

RESULTS

In the group with high dosage of ketamine, the levels of NRG1 and ErbB4 mRNA were significantly lower than that of the group with saline.

CONCLUSION

Ketamine may induce signs similar to schizophrenia in KM mice. The mechanism may be involved in the reduction of NRG1 and ErbB4 mRNA expression.

摘要

目的

探讨氯胺酮致小鼠精神分裂症样行为与海马组织中神经调节蛋白1（NRG1）及表皮生长因子受体4（ErbB4）mRNA表达的相关性，以阐释精神分裂症可能的发病机制。

方法

将50只KM小鼠随机分为5组，分别腹腔注射生理盐水、氯氮平及不同剂量氯胺酮。氯胺酮组按低剂量（25 mg/kg）、中剂量（50 mg/kg）、高剂量（100 mg/kg）腹腔注射，每日1次，连续7 d。高剂量氯胺酮组腹腔注射100 mg/kg氯胺酮7 d后，氯氮平组灌胃给予氯氮平20 mg/kg，每日1次，连续7 d。苏木精-伊红（HE）染色观察海马神经元病理变化；逆转录聚合酶链反应（RT-PCR）检测海马组织中NRG1及ErbB4 mRNA表达。