Karmanskiĭ I M
Vopr Med Khim. 1977 Jul-Aug;23(4):530-4.
Native lipoproteins of low density from human blood serum were intensively hydrolyzed by pepsin in 0.1 M solution of beta-alanine-HCl at pH 1.9. About 50% and 90% of all the protein part of the substances were split off within 60 min and 22 hrs, respectively. Analytical ultracentrifugation showed that the proteolysis occurred in several steps with formation of discrete intermediates (sedimentation coefficients 2.9 S and 2.3 S at solution density 1,012 g/ml).
人血清中的天然低密度脂蛋白在pH 1.9的0.1 M β-丙氨酸-HCl溶液中被胃蛋白酶强烈水解。在60分钟和22小时内，分别约有50%和90%的物质蛋白质部分被分解。分析超速离心表明，蛋白水解分几个步骤进行，形成离散的中间体（溶液密度为1.012 g/ml时，沉降系数分别为2.9 S和2.3 S）。
