National Center for Epidemiology, Budapest, Hungary.
Foodborne Pathog Dis. 2010 May;7(5):507-13. doi: 10.1089/fpd.2009.0420.
In this study, we characterized 110 strains of Salmonella enterica serovar Bovismorbificans contaminating environment, animals, food of animal origin, and human, to assess their significance along the food chain in Hungary. Additionally, five strains from Germany were tested for comparative purposes. Characterization involved antibiotic susceptibility testing, class 1 integron detection by polymerase chain reaction, plasmid profiling, virulotyping (using virulence gene-specific polymerase chain reactions), and pulsed-field gel electrophoresis. Pathogenic potential of selected strains was tested in orally infected 1-day-old specific pathogen-free chicks. Eighty-two percent of the strains were susceptible to the 16 antibiotics tested, and none of them had class 1 integron. A multidrug-resistant human isolate harbored a bla(SHV5)-type extended-spectrum beta-lactamase gene, first reported in this serotype. All the strains possessed avrA, ssaQ, mgtC, spi4, and sopB genes indicating the presence of Salmonella pathogenicity islands 1-5, respectively, missed the phage-related genes sopE and gipA, but retained the phage-related gene sodC1. An approximately 90 kb large plasmid was characteristic to 80% of the strains, all of which carried the spvC gene. In vivo colonization testing of four selected strains in 1-day-old chicks resulted in significantly reduced liver and spleen colonization ability as compared with the Salmonella Enteritidis control strain, whereas their caecal colonization ability differed less from that of Salmonella Enteritidis. Pulsed-field gel electrophoresis data revealed the dominance of two pulsotypes (C2 and C5) without any specific temporal, geographical, and/or source-related linkages. The results show that Salmonella Bovismorbificans studied here are less invasive than Salmonella Enteritidis, but they may colonize and persist in several animal species and successfully contaminate meat products of different animal origin in Hungary.
在这项研究中，我们对污染环境、动物、动物源性食品和人类的 110 株牛型布氏沙门氏菌进行了特征描述，以评估它们在匈牙利食物链中的重要性。此外，还测试了来自德国的 5 株菌株进行比较。特征描述包括抗生素敏感性测试、聚合酶链反应检测 1 类整合子、质粒谱分析、毒力定型（使用毒力基因特异性聚合酶链反应）和脉冲场凝胶电泳。选择的菌株的致病性在 1 日龄无菌小鸡中进行了口服感染测试。82%的菌株对 16 种测试抗生素敏感，均无 1 类整合子。一株多药耐药的人源分离株携带 bla(SHV5)-型超广谱β-内酰胺酶基因，这是首次在该血清型中报道。所有菌株均携带 avrA、ssaQ、mgtC、spi4 和 sopB 基因，分别表明存在沙门氏菌致病性岛 1-5，缺失噬菌体相关基因 sopE 和 gipA，但保留了噬菌体相关基因 sodC1。约 90kb 的大质粒是 80%菌株的特征，所有菌株均携带 spvC 基因。在 1 日龄小鸡体内选择的 4 株菌株的定植试验表明，与肠炎沙门氏菌对照菌株相比，其肝脏和脾脏定植能力显著降低，而其盲肠定植能力与肠炎沙门氏菌的差异较小。脉冲场凝胶电泳数据显示，没有任何特定的时间、地理和/或来源相关联系，有两种优势脉冲型（C2 和 C5）。结果表明，本研究中的牛型布氏沙门氏菌的侵袭性比肠炎沙门氏菌弱，但它们可能在几种动物物种中定植和持续存在，并成功污染匈牙利不同动物源性肉类产品。
