NF-κB 信号蛋白 p50/p105、p52/p100、RelA 和 IKKε 在食管鳞状细胞癌中过表达。

NF-kappaB signalling proteins p50/p105, p52/p100, RelA, and IKKepsilon are over-expressed in oesophageal squamous cell carcinomas.

机构信息

Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.

出版信息

Pathology. 2009;41(7):622-5. doi: 10.3109/00313020903257756.

DOI:10.3109/00313020903257756

PMID:20001340

Abstract

AIMS

Nuclear factor-kappa B (NF-kappaB) activation has been recognised as an important mechanism in the development of cancers. However, expression status of NF-kappaB-related proteins in oesophageal squamous cell carcinoma (ESCC) tissues is largely unknown. In this study, we analysed expressions of NF-kappaB members (p50/105, p52/p100 and RelA) and IKKepsilon in ESCC tissues.

METHODS

We analysed the expression of p50/105, p52/p100, RelA and IKKepsilon in 58 ESCC patients' tissues by immunohistochemistry using a tissue microarray (TMA) method.

RESULTS

Normal oesophageal squamous cells expressed p50/105, p52/p100 and RelA in 5%, 79% and 10% of the tissues in cytoplasm, respectively; however, only p52/p100 was expressed in the nuclei (12%). The cancer tissues expressed p50/105, p52/p100 and RelA in 93%, 95% and 95% in cytoplasm and/or nuclei, respectively. Nuclear immunostainings of NF-kappaB members p50/105, p52/p100 and RelA, which are considered activation of NF-kappaB signalling, were observed in 34%, 60% and 26% of the cancers, respectively. IKKepsilon is expressed in cytoplasm in 50% of the normal squamous tissues and 84% of the cancer tissues. However, none of the expression of p50/105, p52/p100, RelA or IKKepsilon was associated with pathological characteristics, including differentiation, depth of invasion and TNM stage.

CONCLUSION

The increased nuclear expressions of p50/105, p52/p100 and RelA as well as increased cytoplasmic expression of IKKepsilon in the ESCC tissues compared to the normal squamous cells suggested that over-expression of these proteins may be related to activation of the NF-kappaB pathway and might play a role in the development of ESCC.

摘要

目的

核因子-κB（NF-κB）的激活已被认为是癌症发展的重要机制。然而，食管鳞状细胞癌（ESCC）组织中 NF-κB 相关蛋白的表达状态在很大程度上尚不清楚。在本研究中，我们分析了 NF-κB 成员（p50/105、p52/p100 和 RelA）和 IKKε在 ESCC 组织中的表达。

方法

我们使用组织微阵列（TMA）方法通过免疫组织化学分析了 58 例 ESCC 患者组织中 p50/105、p52/p100、RelA 和 IKKε的表达。

结果

正常食管鳞状细胞在细胞质中分别表达 p50/105、p52/p100 和 RelA，阳性率为 5%、79%和 10%；然而，仅 p52/p100 在核内表达（12%）。癌症组织在细胞质和/或核内分别表达 p50/105、p52/p100 和 RelA，阳性率为 93%、95%和 95%。NF-κB 信号转导激活的 NF-κB 成员 p50/105、p52/p100 和 RelA 的核免疫染色分别在 34%、60%和 26%的癌症中观察到。IKKε在 50%的正常鳞状组织和 84%的癌症组织中表达于细胞质中。然而，p50/105、p52/p100、RelA 或 IKKε 的表达均与病理特征（包括分化、浸润深度和 TNM 分期）无关。