Department of Bio-functional Science, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Free Radic Res. 2010 Mar;44(3):267-74. doi: 10.3109/10715760903456100.
Inhibitory effects of intravenously or orally administered antioxidants on the anthralin-derived radical generated in skin (mainly in the epidermis) of living mice by ultraviolet-A (UVA) irradiation were estimated. Anthralin was applied to the dorsal skin of living mice and the mice were then exposed to UVA. The EPR signal intensity in skin tissue strips obtained from mice after anthralin-UVA treatment was measured by an X-band EPR spectrometer. Several common antioxidants such as ascorbate, glutathione and Trolox (a vitamin E analogue) intravenously administered to mice reduced anthralin-derived radical generation. Trolox showed the most prolonged and powerful effect. Intravenous injection of a clinically used cerebral neuroprotective drug, Edarabone (Radicut), also showed depletion for the anthralin-derived radical. Oral administration of a commercialized nutritional supplement (a cocktail of 17 herbals and vitamins) also attenuated the anthralin-derived radical. The anthralin-UVA treatment model for antioxidant activity in the epidermis is a potentially feasible method to estimate activity of antioxidants in the body.
评估了静脉内或口服给予抗氧化剂对紫外线 A（UVA）照射活体小鼠皮肤（主要是表皮）中蒽林衍生自由基的抑制作用。将蒽林应用于活体小鼠的背部皮肤，然后使小鼠暴露于 UVA 下。用 X 波段 EPR 光谱仪测量从蒽林-UVA 处理后的小鼠皮肤组织条中获得的 EPR 信号强度。静脉内给予小鼠的几种常见抗氧化剂，如抗坏血酸、谷胱甘肽和 Trolox（维生素 E 类似物），可减少蒽林衍生自由基的生成。Trolox 显示出最持久和最强的效果。静脉注射一种临床使用的脑神经保护药物 Edarabone（Radicut）也显示出对蒽林衍生自由基的消耗。商业化营养补充剂（17 种草药和维生素的混合物）的口服给药也能减弱蒽林衍生自由基。表皮中抗氧化剂活性的蒽林-UVA 处理模型是一种评估体内抗氧化剂活性的可行方法。
