Department of Biomedical Engineering, Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
Diabetes Technol Ther. 2009 Dec;11(12):805-11. doi: 10.1089/dia.2009.0068.
Transplantation of pancreatic islets has been extensively investigated as a strategy for glycemic control in experimental animals and in patients with diabetes. We investigated whether islet transplantation allows us to obtain adequate islet function during glucose stimulation using a continuous glucose monitoring system (CGMS) in the rat.
We investigated four groups of eight rats each: healthy rats (controls), rats with diabetes, and rats with diabetes transplanted with microencapsulated islets in the peritoneal cavity or transplanted with free islets under the kidney capsule. Syngeneic islets were isolated from Lewis rats. After diabetes induction and islet implantation, when glycemia was stable, a glucose sensor was implanted, and an intraperitoneal glucose tolerance test (IPGTT) was performed to evaluate islet function. Interstitial glucose levels were analyzed, using a theoretical model, to estimate kinetics of glucose metabolism.
Islet transplantation was effective in inducing normoglycemia in both groups, but results of IPGTTs showed that in animals with islets transplanted in microcapsules values of area under the curve and total glucose elimination constant (k(tot)) were significantly different from those in control animals and that these differences were even more important in animals with islets implanted under the kidney capsule.
Our present investigation demonstrates that the application of CGMS was effective in evaluation of glucose metabolism by islet transplantation and indicates that efficient diabetes control can be achieved with this technology.
胰岛移植已被广泛研究作为一种控制实验动物和糖尿病患者血糖的策略。我们研究了胰岛移植是否允许我们在大鼠中使用连续血糖监测系统 (CGMS) 在葡萄糖刺激期间获得足够的胰岛功能。
我们研究了每组 8 只大鼠的四个组：健康大鼠（对照组）、糖尿病大鼠和腹腔内微囊化胰岛移植或肾包膜下自由胰岛移植的糖尿病大鼠。同种胰岛从 Lewis 大鼠中分离出来。在诱导糖尿病和胰岛移植后，当血糖稳定时，植入葡萄糖传感器，并进行腹腔内葡萄糖耐量试验 (IPGTT) 以评估胰岛功能。使用理论模型分析间质葡萄糖水平，以估计葡萄糖代谢动力学。
胰岛移植在两组中均有效诱导正常血糖，但 IPGTT 结果表明，在微囊化胰岛移植的动物中，曲线下面积和总葡萄糖消除常数 (k(tot)) 的值与对照组动物明显不同，而在肾包膜下植入胰岛的动物中这些差异更为重要。
我们目前的研究表明 CGMS 的应用在胰岛移植评估葡萄糖代谢方面是有效的，并表明该技术可以实现有效的糖尿病控制。
