Department of Entomology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Insect Mol Biol. 2010 Apr;19(2):205-15. doi: 10.1111/j.1365-2583.2009.00959.x. Epub 2009 Dec 1.
AeSCP-2, a sterol carrier protein, is involved in sterol trafficking in mosquitoes. The activity of the AeSCP-2 gene is important for mosquito development. An earlier study demonstrated that the transcription of this gene was upregulated by 20-hydroxyecdysone (20E) in cultured gut tissues. To investigate 20E-regulated transcription of the AeSCP-2 gene we truncated the upstream flanking region of AeSCP-2 gene and linked it to a reporter gene. The mosquito Aag-2 cell line was transfected with these promoter/reporter constructs and treated with 20E at various concentrations. Expression vectors of different transcription factors such as HR3 and beta FTZ-F1 were also co-transfected with the AeSCP-2 promoter/reporter constructs. The observed results demonstrated that varied combinations of transcription factors produce different promoter activities of the AeSCP-2 gene. This observation leads us to the conclusion that the partnership of transcription factors is crucial in regulating the transcriptional activity of the AeSCP-2 gene.
AeSCP-2，一种甾醇载体蛋白，参与蚊子中的甾醇运输。AeSCP-2 基因的活性对蚊子的发育很重要。早期的一项研究表明，这种基因的转录受培养的肠道组织中 20-羟基蜕皮酮 (20E) 的上调。为了研究 20E 对 AeSCP-2 基因的转录调控，我们截短了 AeSCP-2 基因的上游侧翼区，并将其与报告基因相连。用这些启动子/报告基因构建体转染蚊子 Aag-2 细胞系，并在不同浓度下用 20E 处理。还将不同转录因子的表达载体（如 HR3 和 beta FTZ-F1）与 AeSCP-2 启动子/报告基因构建体共转染。观察到的结果表明，转录因子的不同组合产生 AeSCP-2 基因的不同启动子活性。这一观察结果使我们得出结论，转录因子的伙伴关系对于调节 AeSCP-2 基因的转录活性至关重要。
