Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Xinfeng, Hsinchu, Taiwan 30401, R.O.C.
Lett Appl Microbiol. 2010 Feb;50(2):198-204. doi: 10.1111/j.1472-765X.2009.02777.x. Epub 2009 Nov 23.
To study the optimization of submerged culture conditions for exopolysaccharide (EPS) production by Armillaria mellea in shake-flask cultures and also to evaluate the performance of an optimized culture medium in a 5-l stirred tank fermenter.
Shake flask cultures for EPS optimal nutritional production contained having the following composition (in g l(-1)): glucose 40, yeast extract 3, KH(2)PO(4) 4 and MgSO(4) 2 at an optimal temperature of 22 degrees C and an initial of pH 4.0. The optimal culture medium was then cultivated in a 5-l stirred tank fermenter at 1 vvm (volume of aeration per volume of bioreactor per min) aeration rate, 150 rev min(-1) agitation speed, controlled pH 4.0 and 22 degrees C. In the optimal culture medium, the maximum EPS production in a 5-l stirred tank fermenter was 588 mg l(-1), c. twice as great as that in the basal medium. The maximum productivity for EPS (Q(p)) and product yield (Y(P/S)) were 42.02 mg l(-1) d(-1) and 26.89 mg g(-1), respectively.
The optimal culture conditions we proposed in this study enhanced the EPS production of A. mellea from submerged cultures.
The optimal culturing conditions we have found will be a suitable starting point for a scale-up of the fermentation process, helping to develop the production of related medicines and health foods from A. mellea.
研究摇瓶培养条件对蜜环菌胞外多糖（EPS）生产的优化，并评估优化培养基在 5 升搅拌罐发酵罐中的性能。
用于 EPS 最佳营养生产的摇瓶培养物含有以下成分（g/L）：葡萄糖 40、酵母提取物 3、KH2PO4 4 和 MgSO4 2，最佳温度为 22°C，初始 pH 值为 4.0。然后，在 5 升搅拌罐发酵罐中以 1 vvm（每分钟每升生物反应器的通气量）通气率、150 rev/min（每分钟搅拌速度）、控制 pH 值为 4.0 和 22°C 培养最佳培养基。在最佳培养基中，5 升搅拌罐发酵罐中 EPS 的最大产量为 588mg/L，约为基础培养基的两倍。EPS 的最大比生产率（Q(p)）和产物产率（Y(P/S)）分别为 42.02mg/L/d 和 26.89mg/g。
本研究提出的最佳培养条件提高了蜜环菌的 EPS 产量。
我们发现的最佳培养条件将成为发酵过程放大的合适起点，有助于从蜜环菌开发相关药品和保健品的生产。
