爱德华氏菌外膜蛋白的蛋白质组学分析。

Proteomic analysis of outer membrane proteins of Edwardsiella tarda.

机构信息

National Bureau of Fish Genetic Resources (ICAR), Lucknow, India.

出版信息

J Appl Microbiol. 2010 Jun;108(6):2214-21. doi: 10.1111/j.1365-2672.2009.04627.x. Epub 2009 Nov 14.

DOI:10.1111/j.1365-2672.2009.04627.x

PMID:20002913

Abstract

AIMS

The purpose of this study was to identify outer membrane proteins (OMPs) of Edwardsiella tarda.

METHODS AND RESULTS

The OMPs from a virulent strain of E. tarda (ET-7) was extracted using lauroyl sarcosine method. The OMPs were analysed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and protein spots were identified using matrix assisted laser desorption/ionization-time-of-flight mass spectrometry. A total of 21 proteins were identified from 24 protein spots observed on the 2D-PAGE gel. These proteins were identified as GroEL, antigenic proteins, ABC transporters, elongation factors, OmpA, PTSINtr with GAF domain, catalase C, glycolytic enzymes, DnaJ, transcriptional regulator, proteins mraZ and ccdA. Subcellular localizations, beta-barrel OMPs and lipoproteins of identified proteins were predicted using PSORTb, PRED-TMBB and LipoP1.0 programme.

CONCLUSIONS

Identification, localization and possible functions of OMPs of E. tarda were studied.

SIGNIFICANCE AND IMPACT OF THE STUDY

These proteins could be used for development of novel drug targets, diagnostics or vaccine against edwardsiellosis.

摘要

目的

本研究旨在鉴定迟缓爱德华氏菌的外膜蛋白（OMPs）。

方法和结果

采用月桂酰肌氨酸法提取强毒株迟缓爱德华氏菌（ET-7）的 OMPs。通过二维聚丙烯酰胺凝胶电泳（2D-PAGE）对 OMPs 进行分析，并使用基质辅助激光解吸/电离飞行时间质谱鉴定蛋白斑点。从 2D-PAGE 凝胶上观察到的 24 个蛋白斑点中鉴定出 21 种蛋白。这些蛋白被鉴定为 GroEL、抗原蛋白、ABC 转运蛋白、延伸因子、OmpA、具有 GAF 结构域的 PTSINtr、过氧化氢酶 C、糖酵解酶、DnaJ、转录调节因子、mraZ 和 ccdA 蛋白。使用 PSORTb、PRED-TMBB 和 LipoP1.0 程序预测鉴定蛋白的亚细胞定位、β-桶 OMPs 和脂蛋白。