Laboratory of Immunohematology and Immunochemistry, New York Blood Center, and the Transfusion Service, St Vincent's Hospital, New York, New York, USA.
Transfusion. 2010 Apr;50(4):856-60. doi: 10.1111/j.1537-2995.2009.02522.x. Epub 2009 Dec 9.
Alloantibodies that define some high-prevalence MNS antigens are made by people with glycophorin A (GPA) altered by a single-amino-acid change or replacement of amino acids from part of the Pseudoexon 3 of GYP*B. The finding of a patient whose plasma contained a novel alloanti-En(a)FR prompted this study.
The patient's serum contained an alloantibody to a high-prevalence antigen, resistant to papain, ficin, trypsin, alpha-chymotrypsin, or dithiothreitol. The antibody was strongly reactive with all panel red blood cells (RBCs) tested, showed reduced reactivity with ENEP- and ENAV- RBCs, and was nonreactive with M(k)M(k), En(a-), GP.Hil/GP.Hil, and GP.JL/M(k) RBCs. The patient's RBCs typed M+N-S+s-, Wr(a-b+(w)), ENEP-, and ENAV-. These results indicated that the antibody recognized a new high-prevalence antigen in the MNS system. Sequencing of DNA prepared from the patient's white blood cells revealed a GYP*A nucleotide substitution of 242T>G (predicted to change Val62 of GPA to Gly). This change ablates an RsaI restriction enzyme site and polymerase chain reaction-restriction fragment length polymorphism confirmed that the proband was homozygous for Nucleotide 242G.
We describe a novel high-prevalence MNS antigen, characterized by Val62 in GPA and named ENEV. The absence of the antigen is associated with Gly62. The change explains the weakened reactivity of the patient's serum with ENEP- and ENAV- RBCs and nonreactivity with anti-ENEP and anti-ENAV against her RBCs. The ENEV antigen has been assigned the ISBT number MNS45.
定义一些高流行 MNS 抗原的同种抗体是由糖蛋白 A (GPA) 发生单一氨基酸改变或部分 Pseudoexon 3 的 GYP*B 氨基酸替换的人产生的。发现一位患者的血浆中含有一种新型 alloanti-En(a)FR，促使进行了这项研究。
患者的血清中含有一种对高流行抗原的同种抗体，对木瓜蛋白酶、ficin、胰蛋白酶、α-糜蛋白酶或二硫苏糖醇具有抗性。该抗体与所有测试的红细胞（RBC）均强烈反应，与 ENEP-和 ENAV-RBC 的反应性降低，与 M(k)M(k)、En(a)-、GP.Hil/GP.Hil 和 GP.JL/M(k) RBC 不反应。患者的 RBC 型为 M+N-S+s-、Wr(a-b+(w))、ENEP-和 ENAV-。这些结果表明，该抗体识别了 MNS 系统中的一种新的高流行抗原。从患者白细胞中提取的 DNA 测序显示 GYP*A 核苷酸取代 242T>G（预测将 GPA 中的 Val62 改变为 Gly）。这种变化消除了 RsaI 限制酶位点，聚合酶链反应-限制片段长度多态性证实了该先证者是核苷酸 242G 的纯合子。
我们描述了一种新的高流行 MNS 抗原，其特征为 GPA 中的 Val62，并命名为 ENEV。该抗原的缺失与 Gly62 相关。该变化解释了患者血清与 ENEP-和 ENAV-RBC 的反应性减弱以及与她 RBC 的抗-ENEP 和抗-ENAV 不反应。ENEV 抗原已被分配 ISBT 编号 MNS45。
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