Laboratorio de Limnología, INIBIOMA, CONICET-UNComahue, Bariloche, Argentina.
Photochem Photobiol. 2010 Mar-Apr;86(2):367-73. doi: 10.1111/j.1751-1097.2009.00675.x. Epub 2009 Dec 14.
We analyzed the effects of UV radiation (UVR) effects on acetylcholinesterase (AChE) activity in two calanoid copepods, Boeckella gibbosa and Parabroteas sarsi that inhabit Patagonian shallow lakes. We studied the effect of experimental UVR (UV-B and UV-A) exposure on AChE activity in relation to basal antioxidant capacities of both copepods. Our experiments showed that UVR can effectively depress AChE activity, although with differences between species. In both copepods AChE was affected by UV-B, whereas UV-A only affected AChE in B. gibbosa. Both copepods also differed in body elemental composition (C:N:P), photoprotecting compound content (carotenoids and mycosporine-like amino acids) and enzymatic antioxidant capacity (glutathione S-transferase [GST]). Our results suggest that when exposed to UVR, AChE activity would depend more on the antioxidant capacity (GST) and P availability for enzyme synthesis than on the photoprotective compounds.
我们分析了紫外线辐射（UVR）对栖息在巴塔哥尼亚浅湖中的两种哲水蚤（Boeckella gibbosa 和 Parabroteas sarsi）乙酰胆碱酯酶（AChE）活性的影响。我们研究了实验 UVR（UV-B 和 UV-A）暴露对两种桡足类动物基础抗氧化能力的 AChE 活性的影响。我们的实验表明，UVR 可以有效抑制 AChE 活性，但不同物种之间存在差异。在这两种桡足类动物中，AChE 均受到 UV-B 的影响，而 UV-A 仅影响 B. gibbosa 的 AChE。两种桡足类动物的身体元素组成（C:N:P）、光保护化合物含量（类胡萝卜素和菌氨酸类似物氨基酸）和酶抗氧化能力（谷胱甘肽 S-转移酶 [GST]）也存在差异。我们的结果表明，当暴露于 UVR 时，AChE 活性将更多地取决于抗氧化能力（GST）和 P 对酶合成的可用性，而不是光保护化合物。
