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用于快速高效测定白细胞介素-6启动子-597/-572/-174单倍型的序列特异性引物优化聚合酶链反应（PCR-SSP）

Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter -597/-572/-174Haplotypes.

作者信息

Müller-Steinhardt Michael, Schulte Friederike, Klüter Harald, Bugert Peter

机构信息

Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Friedrich-Ebert-Strasse 107, 68167 Mannheim, Germany, DRK-Blutspendedienst Baden-Württemberg - Hessen, Germany.

出版信息

BMC Res Notes. 2009 Dec 10;2:245. doi: 10.1186/1756-0500-2-245.

DOI:10.1186/1756-0500-2-245
PMID:20003261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2797812/
Abstract

BACKGROUND

Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G-->A), -572(G-->C) and -174(G-->C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 -597/-572/-174haplotyping are elaborate and require large amounts of genomic DNA.

FINDINGS

We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions.

CONCLUSION

This enables a fast and efficient determination of IL-6 -597/-572/-174haplotypes in clinical diagnosis and further evaluation of IL-6 promoter polymorphisms in larger patient cohorts.

摘要

背景

白细胞介素-6（IL-6）启动子在-597（G→A）、-572（G→C）和-174（G→C）位置的多态性已显示对不同的主要疾病有临床影响。目前用于IL-6 -597/-572/-174单倍型分型的PCR-SSP方案很复杂，需要大量的基因组DNA。

研究结果

我们描述了一种改进的分型技术，由于优化了PCR条件，该技术所需的PCR反应数量减少且PCR运行时间缩短。

结论

这使得在临床诊断中能够快速、高效地确定IL-6 -597/-572/-174单倍型，并在更大的患者队列中进一步评估IL-6启动子多态性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd22/2797812/9fa9855276f6/1756-0500-2-245-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd22/2797812/9fa9855276f6/1756-0500-2-245-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd22/2797812/9fa9855276f6/1756-0500-2-245-1.jpg

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