Rab1和Rab6 GTP酶在哺乳动物视网膜中的细胞类型特异性和光依赖性表达。
Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.
作者信息
Huang Wei, Wu Guangyu, Wang Guo-Yong
机构信息
Department of Structural and Cellular Biology, School of Medicine, Tulane University, New Orleans, Louisiana 70112, USA.
出版信息
Vis Neurosci. 2009 Nov;26(5-6):443-52. doi: 10.1017/S0952523809990277. Epub 2009 Dec 11.
Abstract
The Ras-like Rab1 and Rab6 GTPases modulate protein traffic along the early secretory pathway and are involved in the regulation of maturation of rhodopsin in the outer retina. However, Rab GTPases have not been studied in the inner retinas. Here, we analyzed the anatomatic distribution and expression of Rab1 and Rab6 in the mouse and rat retinas by immunohistochemistry and immunoblotting. We found that Rab1 was specifically expressed in the rod bipolar cells, while Rab6 was expressed in a different cell type(s) from rod bipolar cells in the inner retina. We also demonstrated that expression of Rab1 and Rab6 was increased with light. These data provided the first evidence implicating that Rab1 and Rab6 may be involved in the regulation of the retinal adaptation.
摘要
类Ras的Rab1和Rab6 GTP酶调节早期分泌途径中的蛋白质运输，并参与视网膜外层视紫红质成熟的调节。然而，Rab GTP酶尚未在内层视网膜中得到研究。在这里，我们通过免疫组织化学和免疫印迹分析了Rab1和Rab6在小鼠和大鼠视网膜中的解剖分布和表达。我们发现Rab1在视杆双极细胞中特异性表达，而Rab6在内层视网膜中表达于与视杆双极细胞不同的细胞类型中。我们还证明Rab1和Rab6的表达随光照增加。这些数据提供了首个证据，表明Rab1和Rab6可能参与视网膜适应性调节。
相似文献
1
Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.Rab1和Rab6 GTP酶在哺乳动物视网膜中的细胞类型特异性和光依赖性表达。
Vis Neurosci. 2009 Nov;26(5-6):443-52. doi: 10.1017/S0952523809990277. Epub 2009 Dec 11.
2
Giantin interacts with both the small GTPase Rab6 and Rab1.巨糖蛋白与小GTP酶Rab6和Rab1均相互作用。
Exp Cell Res. 2007 Jul 1;313(11):2318-25. doi: 10.1016/j.yexcr.2007.03.031. Epub 2007 Apr 1.
3
Identification and characterization of interacting partners of Rab GTPases by yeast two-hybrid analyses.通过酵母双杂交分析鉴定和表征Rab GTP酶的相互作用伙伴。
Methods Mol Biol. 2008;440:111-25. doi: 10.1007/978-1-59745-178-9_9.
4
The fungal RABOME: RAB GTPases acting in the endocytic and exocytic pathways of Aspergillus nidulans (with excursions to other filamentous fungi).真菌 RABOME：在构巢曲霉（Aspergillus nidulans）的内吞和外排途径中发挥作用的 RAB GTPases（也涉及其他丝状真菌）。
Mol Microbiol. 2021 Jul;116(1):53-70. doi: 10.1111/mmi.14716. Epub 2021 Apr 3.
5
Regulation of anterograde transport of adrenergic and angiotensin II receptors by Rab2 and Rab6 GTPases.Rab2和Rab6 GTP酶对肾上腺素能和血管紧张素II受体顺向运输的调节
Cell Signal. 2007 Nov;19(11):2388-99. doi: 10.1016/j.cellsig.2007.07.017. Epub 2007 Aug 1.
6
Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts with multiple Rab GTPases.肺炎衣原体包涵体膜蛋白Cpn0585与多种Rab GTP酶相互作用。
Infect Immun. 2007 Dec;75(12):5586-96. doi: 10.1128/IAI.01020-07. Epub 2007 Oct 1.
7
Regulation of the cell surface expression and function of angiotensin II type 1 receptor by Rab1-mediated endoplasmic reticulum-to-Golgi transport in cardiac myocytes.Rab1介导的内质网到高尔基体转运对心肌细胞中血管紧张素II 1型受体细胞表面表达和功能的调节
J Biol Chem. 2004 Sep 24;279(39):41077-84. doi: 10.1074/jbc.M405988200. Epub 2004 Jul 12.
8
Restoration of Rab1 Levels Prevents Endoplasmic Reticulum Stress in Hippocampal Cells during Protein Aggregation Triggered by Rotenone.在鱼藤酮引发的蛋白质聚集过程中，Rab1 水平的恢复可防止海马细胞内质网应激。
Neuroscience. 2019 Nov 1;419:5-13. doi: 10.1016/j.neuroscience.2019.08.050. Epub 2019 Sep 3.
9
Two-hybrid system screen with the small GTP-binding protein Rab6. Identification of a novel mouse GDP dissociation inhibitor isoform and two other potential partners of Rab6.
J Biol Chem. 1995 Jun 16;270(24):14801-8. doi: 10.1074/jbc.270.24.14801.
10
Diversity and plasticity in Rab GTPase nucleotide release mechanism has consequences for Rab activation and inactivation.Rab GTP 酶核苷酸释放机制的多样性和可塑性对 Rab 的激活和失活具有影响。
Elife. 2014 Feb 11;3:e01623. doi: 10.7554/eLife.01623.
引用本文的文献
1
Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9).Rab6A作为小鼠和人类大脑中的泛星形胶质细胞标志物，并与其他胶质细胞标志物（GFAP、GS、Aldh1L1、SOX9）进行比较。
Cells. 2021 Jan 5;10(1):72. doi: 10.3390/cells10010072.
2
GCAP1, Rab6, and HSP27: Novel Autoantibody Targets in Cancer-Associated Retinopathy and Autoimmune Retinopathy.GCAP1、Rab6和HSP27：癌症相关性视网膜病变和自身免疫性视网膜病变中的新型自身抗体靶点。
Transl Vis Sci Technol. 2016 May 2;5(3):1. doi: 10.1167/tvst.5.3.1. eCollection 2016 May.
3
Rab1 in cell signaling, cancer and other diseases.细胞信号传导、癌症及其他疾病中的Rab1
Oncogene. 2016 Nov 3;35(44):5699-5704. doi: 10.1038/onc.2016.81. Epub 2016 Apr 4.
本文引用的文献
1
Glutamate metabolic pathways and retinal function.谷氨酸代谢途径与视网膜功能。
J Neurochem. 2009 Oct;111(2):589-99. doi: 10.1111/j.1471-4159.2009.06354.x. Epub 2009 Aug 22.
2
The sensitivity of light-evoked responses of retinal ganglion cells is decreased in nitric oxide synthase gene knockout mice.在一氧化氮合酶基因敲除小鼠中，视网膜神经节细胞的光诱发反应敏感性降低。
J Vis. 2007 Nov 30;7(14):7.1-13. doi: 10.1167/7.14.7.
3
Regulation of anterograde transport of adrenergic and angiotensin II receptors by Rab2 and Rab6 GTPases.Rab2和Rab6 GTP酶对肾上腺素能和血管紧张素II受体顺向运输的调节
Cell Signal. 2007 Nov;19(11):2388-99. doi: 10.1016/j.cellsig.2007.07.017. Epub 2007 Aug 1.
4
The impact of photoreceptor noise on retinal gain controls.光感受器噪声对视网膜增益控制的影响。
Curr Opin Neurobiol. 2006 Aug;16(4):363-70. doi: 10.1016/j.conb.2006.06.013. Epub 2006 Jul 11.
5
Differential regulation of the cell-surface targeting and function of beta- and alpha1-adrenergic receptors by Rab1 GTPase in cardiac myocytes.Rab1 GTP酶对心肌细胞中β-和α1-肾上腺素能受体的细胞表面靶向及功能的差异调节
Mol Pharmacol. 2006 May;69(5):1571-8. doi: 10.1124/mol.105.019984. Epub 2006 Feb 3.
6
Retinal network adaptation to bright light requires tyrosinase.视网膜网络对强光的适应需要酪氨酸酶。
Nat Neurosci. 2004 Dec;7(12):1329-36. doi: 10.1038/nn1344. Epub 2004 Oct 31.
7
Regulation of the cell surface expression and function of angiotensin II type 1 receptor by Rab1-mediated endoplasmic reticulum-to-Golgi transport in cardiac myocytes.Rab1介导的内质网到高尔基体转运对心肌细胞中血管紧张素II 1型受体细胞表面表达和功能的调节
J Biol Chem. 2004 Sep 24;279(39):41077-84. doi: 10.1074/jbc.M405988200. Epub 2004 Jul 12.
8
Distinct pathways for the trafficking of angiotensin II and adrenergic receptors from the endoplasmic reticulum to the cell surface: Rab1-independent transport of a G protein-coupled receptor.血管紧张素II和肾上腺素能受体从内质网运输至细胞表面的不同途径：G蛋白偶联受体的不依赖Rab1的运输
J Biol Chem. 2003 Nov 21;278(47):47062-9. doi: 10.1074/jbc.M305707200. Epub 2003 Sep 11.
9
A comparative study of protein kinase C-like immunoreactive cells in the retina.
Brain Behav Evol. 2000 Dec;56(6):330-9. doi: 10.1159/000047217.
10
Adaptation in vertebrate photoreceptors.脊椎动物光感受器中的适应性。
Physiol Rev. 2001 Jan;81(1):117-151. doi: 10.1152/physrev.2001.81.1.117.
Zotero 插件