Yan Jian-ying, Zhang Dong-mei
Department of Obstetrics and Gynecology, Fujian Maternity and Child Health Hospital, Fuzhou 350001, China.
Zhonghua Fu Chan Ke Za Zhi. 2009 Aug;44(8):578-82.
To determine the expression of adiponectin mRNA in omental and subcutaneous adipose tissues and serum levels of adiponectin in pregnant women complicated with pre-eclampsia (PE) in order to find out its association with the pathogenesis of PE.
The expression of adiponectin mRNA in omental and subcutaneous adipose tissue of 49 women with severe PE, which were divided into early-onset group (n = 24, presented at <or= 34 weeks of gestation) and late-onset group (n = 25, presented at > 34 weeks of gestation), and 30 healthy pregnant women (control), who presented to Fujian Maternity and Child Health Hospital for prenatal care from September 2007 to July 2008, were determined by real-time fluorescent quantitative reverse transcription PCR. The maternal serum level of adiponectin was detected by enzyme-linked immunosorbent assay for all subjects.
(1) The levels of serum adiponectin in the early-onset and late-onset severe PE groups were (5.9 +/- 2.7) and (7.9 +/- 2.8) mg/L, which were significantly lower than that of the control (11.2 +/- 4.2) mg/L, respectively (P < 0.01), and the level in the early-onset group was lower than in the late-onset group (P < 0.05). (2) Among the early-onset, late-onset PE group and the controls, significant difference was found between any two groups of the three in the adiponectin mRNA expression in omental adipose tissue (0.56 +/- 0.17, 0.69 +/- 0.23 and 1.00 +/- 0.22, P < 0.01), but not in the subcutaneous adipose tissue (0.90 +/- 0.19, 0.93 +/- 0.23 and 1.00 +/- 0.16, P > 0.05). (3) The adiponectin mRNA expressions in the subcutaneous and omental adipose tissue were positively correlated with the maternal serum level of adiponectin in all groups (P < 0.05). (4) The omental adiponectin mRNA levels were negatively correlated with maternal serum triglyceride levels in all of the three groups (r = -0.610, -0.659, -0.446, respectively, and all P < 0.05). Negative correlation was also found between the omental adiponectin mRNA level and the prepregnant body mass index in the control group (r = -0.436, P < 0.05).
Decreased maternal serum adiponectin level may be involved in the pathogenesis of pre-eclampsia and also may contribute to the reduced expression of adiponectin in the omental adipose tissue.
检测子痫前期（PE）孕妇网膜及皮下脂肪组织中脂联素mRNA的表达及血清脂联素水平，以探讨其与PE发病机制的关系。
选取2007年9月至2008年7月在福建省妇幼保健院产检的49例重度PE孕妇，分为早发型组（n = 24，孕龄≤34周）和晚发型组（n = 25，孕龄>34周），另选30例健康孕妇作为对照组。采用实时荧光定量逆转录PCR法检测各组孕妇网膜及皮下脂肪组织中脂联素mRNA的表达，采用酶联免疫吸附法检测所有研究对象的血清脂联素水平。
（1）早发型和晚发型重度PE组孕妇血清脂联素水平分别为（5.9±2.7）mg/L和（7.9±2.8）mg/L，均显著低于对照组的（11.2±4.2）mg/L（P<0.01），且早发型组低于晚发型组（P<0.05）。（2）早发型、晚发型PE组和对照组三组间网膜脂肪组织脂联素mRNA表达两两比较差异均有统计学意义（分别为0.56±0.17、0.69±0.23和1.00±0.22，P<0.01），而皮下脂肪组织差异无统计学意义（分别为0.90±0.19、0.93±0.23和1.00±0.16，P>0.05）。（3）各组皮下及网膜脂肪组织脂联素mRNA表达均与孕妇血清脂联素水平呈正相关（P<0.05）。（4）三组孕妇网膜脂联素mRNA水平均与血清甘油三酯水平呈负相关（r值分别为-0.610、-0.659、-0.446，均P<0.05），对照组网膜脂联素mRNA水平与孕前体质指数也呈负相关（r = -0.436，P<0.05）。
孕妇血清脂联素水平降低可能参与了子痫前期的发病机制，也可能导致网膜脂肪组织中脂联素表达减少。
