Li Xiao-feng, Lu Hong-yun, Jiang Wei, Qiu Ya-wei, Zeng Long-yi
Department of Endocrinology & Metabolism, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
Zhonghua Yi Xue Za Zhi. 2012 May 29;92(20):1388-91.
To explore the depot-specific mRNA and protein expression of caveolin-1 (CAV-1) in human subcutaneous and omental adipose tissues and analyze their relationship with obesity and insulin resistance.
A total of 41 subjects with normal glucose regulation were recruited. Among them, there were 29 cases with normal body mass index (BMI) and 12 cases with BMI ≥ 24 kg/m(2). All were scheduled to undergone selective abdominal operations. The levels of fasting insulin (FINS) were measured by chemiluminescence enzyme-linked immunosorbent assay (ELISA) kit. Fasting plasma glucose (FPG) was tested by glucose oxidase and home model insulin resistance index (HOMA-IR) calculated. Real-time polymerase chain reaction (RT-PCR) and Western blotting were employed to assess the relative mRNA and protein expression of caveolin-1 in subcutaneous and omental adipose tissues. And the mRNA and protein expression of caveolin-1 from different fat depots were compared and their correlations with BMI and HOMA-IR analyzed.
(1) FINS and HOMA-IR were significantly higher in the overweight and obesity group than those in the normal BMI group (FINS: (8.82 ± 3.79) mU/L vs (6.43 ± 4.38) mU/L, P < 0.05, HOMA-1R: 1.91 ± 0.85 vs 1.36 ± 0.72, P < 0.05). (2) The normal BMI group patients had the higher expression levels of caveolin-1 mRNA in omental adipose tissue than overweight counterparts (2.84 ± 0.86 vs 1.02 ± 0.36, P < 0.01). But the difference in subcutaneous adipose tissue was not significant (P > 0.05). (3) The caveolin-1 protein expression in omental adipose tissue of the normal BMI group was higher than that of overweight patients (1.68 ± 0.67 vs 0.73 ± 0.29, P < 0.05). And the difference between two groups was not significant (P > 0.05). (4)The expressions of caveolin-1 mRNA in omental adipose tissue were negatively correlated with BMI, waist circumstance, triglyceride and HOMA-IR (r = -0.441, -0.615, -0.539, -0.688, P < 0.05). No correlations were found between the expressions of caveolin-1 mRNA in subcutaneous adipose tissue with BMI, waist circumstance and HOMA-IR (P > 0.05).
Differential depot-specific expressions of caveolin-1 are present in human subcutaneous and omental adipose tissues. A low expression of caveolin-1 in omental adipose tissue may contribute to the pathogenesis of obesity and insulin resistance.
探讨小窝蛋白-1（CAV-1）在人皮下和网膜脂肪组织中特定部位的mRNA和蛋白表达情况，并分析其与肥胖及胰岛素抵抗的关系。
招募41例糖调节正常的受试者。其中，29例体重指数（BMI）正常，12例BMI≥24kg/m²。所有受试者均计划接受选择性腹部手术。采用化学发光酶联免疫吸附测定（ELISA）试剂盒检测空腹胰岛素（FINS）水平。用葡萄糖氧化酶法检测空腹血糖（FPG），并计算稳态模型胰岛素抵抗指数（HOMA-IR）。采用实时聚合酶链反应（RT-PCR）和蛋白质印迹法评估皮下和网膜脂肪组织中小窝蛋白-1的相对mRNA和蛋白表达。比较不同脂肪部位小窝蛋白-1的mRNA和蛋白表达，并分析其与BMI和HOMA-IR的相关性。
（1）超重和肥胖组的FINS和HOMA-IR显著高于正常BMI组（FINS：（8.82±3.79）mU/L对（6.43±4.38）mU/L，P<0.05；HOMA-1R：1.91±0.85对1.36±0.72，P<0.05）。（2）正常BMI组患者网膜脂肪组织中小窝蛋白-1 mRNA表达水平高于超重患者（2.84±0.86对1.02±0.36，P<0.01）。但皮下脂肪组织差异无统计学意义（P>0.05）。（3）正常BMI组网膜脂肪组织中小窝蛋白-1蛋白表达高于超重患者（1.68±0.67对0.73±0.29，P<0.05）。两组间差异无统计学意义（P>0.05）。（4）网膜脂肪组织中小窝蛋白-1 mRNA表达与BMI、腰围、甘油三酯和HOMA-IR呈负相关（r=-0.441、-0.615、-0.539、-0.688，P<0.05）。皮下脂肪组织中小窝蛋白-1 mRNA表达与BMI、腰围和HOMA-IR之间无相关性（P>0.05）。
人皮下和网膜脂肪组织存在小窝蛋白-1特定部位的差异表达。网膜脂肪组织中小窝蛋白-1低表达可能参与肥胖和胰岛素抵抗的发病机制。
