Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
Cell Mol Immunol. 2009 Dec;6(6):433-40. doi: 10.1038/cmi.2009.55.
Non-human primates such as Chinese rhesus macaques are the favorable models for preclinical study of potential therapeutic drugs, vaccines and mechanisms of human diseases. Little is known about the normal levels of leukocyte subpopulations of Chinese rhesus macaques. To obtain these data, 100 blood samples from Chinese rhesus macaques were collected. The normal range of major leukocyte subpopulations, such as T lymphocytes, B lymphocytes, monocytes, myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs), were quantitatively analyzed by flow cytometry through BD trucount tubes. The influence of age and sex on the cell counts of leukocyte subpopulations was analyzed. The counts of CD3(+) T cells, CD3(+)CD4(+) T cells, CD3(+)CD8(+) T cells and B cells decreased with age, but those of monocytes, mDCs and pDCs had no significant correlation with age. Significant differences existed in the cell counts of most leukocyte subpopulations between the male and female groups except pDCs. And the values of the females were higher than those of the males. The study provided basic information about the leukocyte subpopulations of Chinese rhesus macaques, and it may be valuable for immunobiological study of Chinese rhesus macaques.
非人类灵长类动物，如食蟹猴，是临床前研究人类疾病潜在治疗药物、疫苗和机制的理想模型。目前对于食蟹猴白细胞亚群的正常水平知之甚少。为了获得这些数据，采集了 100 份食蟹猴血液样本。通过 BD trucount 管，采用流式细胞术对主要白细胞亚群（如 T 淋巴细胞、B 淋巴细胞、单核细胞、髓系树突状细胞(mDCs)和浆细胞样树突状细胞(pDCs)）的正常范围进行定量分析。分析了年龄和性别对白细胞亚群细胞计数的影响。CD3(+)T 细胞、CD3(+)CD4(+)T 细胞、CD3(+)CD8(+)T 细胞和 B 细胞的计数随年龄增长而减少，但单核细胞、mDCs 和 pDCs 的计数与年龄无明显相关性。除了 pDCs 之外，大多数白细胞亚群在男性和女性群体之间的细胞计数存在显著差异。而且女性的数值高于男性。该研究提供了食蟹猴白细胞亚群的基本信息，可能对食蟹猴的免疫生物学研究具有重要价值。
