Veazey R S, Rosenzweig M, Shvetz D E, Pauley D R, DeMaria M, Chalifoux L V, Johnson R P, Lackner A A
Division of Comparative Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA.
Clin Immunol Immunopathol. 1997 Mar;82(3):230-42. doi: 10.1006/clin.1996.4318.
This study characterizes the gut-associated lymphoid tissue (GALT) of normal healthy rhesus macaques and compares the percentages of T and B cell subsets to those of systemic lymphoid tissue. Lymphocytes from the systemic lymphoid tissue (spleen, axillary, and inguinal lymph nodes), mesenteric lymph nodes (MLN), and intestinal epithelium (IEL) and lamina propria (LPL) of the jejunum, ileum, and colon were examined from both adult and juvenile, normal rhesus macaques. Lymphocytes were analyzed for expression of CD2, CD3, CD4, CD8, CD25, gamma delta TCR, and CD20 by two- or three-color flow cytometric analysis. Sections of jejunum, ileum, and colon were examined for CD3, CD20, and CD103 expression by immunohistochemistry. Peyer's patches were also examined for CD3, CD4, CD8, and CD20 expression by immunohistochemistry. Most IEL and LPL were CD103+, CD3+ T cells with significantly fewer CD20+ B cells. The IEL were predominantly CD3+CD8+ (63-80%), with very few CD4+ cells, whereas CD4:CD8 ratios in the LPL ranged from 0.74 to 1.3. Three to 38% of the IEL were gamma delta TCR positive, but gamma delta expression was rare in the LPL and MLN. gamma delta TCR expression was also higher in the IEL of younger animals. LPL had higher expression of CD25 compared to IEL and systemic tissues, particularly in aged animals. CD4+CD8+, double-positive and CD3+CD4-CD8- double-negative cells were also observed in GALT. These results demonstrate that GALT of rhesus macaques is remarkably similar to that of humans, further justifying the use of these animals as models for various intestinal disorders.
本研究对正常健康恒河猴的肠道相关淋巴组织（GALT）进行了表征，并将T和B细胞亚群的百分比与全身淋巴组织的进行了比较。从成年和幼年正常恒河猴中检查了来自全身淋巴组织（脾脏、腋窝和腹股沟淋巴结）、肠系膜淋巴结（MLN）以及空肠、回肠和结肠的肠上皮（IEL）和固有层（LPL）的淋巴细胞。通过双色或三色流式细胞术分析淋巴细胞的CD2、CD3、CD4、CD8、CD25、γδTCR和CD20表达。通过免疫组织化学检查空肠、回肠和结肠切片的CD3、CD20和CD103表达。还通过免疫组织化学检查派尔集合淋巴结的CD3、CD4、CD8和CD20表达。大多数IEL和LPL是CD103+、CD3+T细胞，CD20+B细胞明显较少。IEL主要是CD3+CD8+（63 - 80%），CD4+细胞很少，而LPL中的CD4:CD8比率在0.74至1.3之间。3%至38%的IEL是γδTCR阳性，但γδ在LPL和MLN中的表达很少。γδTCR在幼年动物的IEL中表达也更高。与IEL和全身组织相比，LPL的CD25表达更高，尤其是在老年动物中。在GALT中也观察到了CD4+CD8+双阳性和CD3+CD4 - CD8 - 双阴性细胞。这些结果表明，恒河猴的GALT与人类的非常相似，进一步证明了将这些动物用作各种肠道疾病模型的合理性。
