Department of Chemical Engineering, National Chung Cheng University, Taiwan, ROC.
Colloids Surf B Biointerfaces. 2010 Mar 1;76(1):286-91. doi: 10.1016/j.colsurfb.2009.11.006. Epub 2009 Nov 13.
This study analyzes the effects of Tween 80 and Span 20 on the electrical interaction between cationic solid lipid nanoparticles (CSLNs) and human brain microvascular endothelial cells (HBMECs). Electrophoretic mobility, zeta potential and fixed charge density of CSLNs and HBMECs with uptake of CSLNs were also investigated. The results revealed that a higher molar ratio of Tween 80 yielded smaller CSLNs, higher charge of CSLNs and larger electrical attraction energy between CSLNs and HBMECs. On the contrary, a decrease in the molar ratio of Tween 80 enhanced the absolute values of electrophoretic mobility and zeta potential of CSLNs-incorporating HBMECs. In addition, a higher concentration of CSLNs in the dispersion medium led to lower charge of CSLNs-incorporating HBMECs. An increased concentration of glutamate from 1 to 2mg/mL enhanced the absolute values of electrophoretic properties of CSLNs-incorporating HBMECs. In the case of increasing concentration of glutamate, pure Tween 80 was stronger in charge enhancement than pure Span 20.
本研究分析了吐温 80 和司盘 20 对阳离子固体脂质纳米粒（CSLNs）与人脑微血管内皮细胞（HBMECs）之间电相互作用的影响。还研究了摄取 CSLNs 后 CSLNs 和 HBMECs 的电泳迁移率、zeta 电位和固定电荷密度。结果表明，较高摩尔比的吐温 80 产生较小的 CSLNs、较高的 CSLNs 电荷和 CSLNs 与 HBMECs 之间更大的电吸引力。相反，吐温 80 的摩尔比降低会增强 CSLNs 结合 HBMECs 的电泳迁移率和 zeta 电位的绝对值。此外，分散介质中 CSLNs 浓度的增加导致 CSLNs 结合 HBMECs 的电荷降低。谷氨酸浓度从 1mg/mL 增加到 2mg/mL 会增强 CSLNs 结合 HBMECs 的电泳性质的绝对值。在增加谷氨酸浓度的情况下，纯吐温 80 比纯司盘 20 更能增强电荷。
