Department of Immunology, Tuberculosis Research Centre, Indian Council of Medical Research, Mayor V.R. Ramanathan Road, Chennai 600 031, India.
Cytokine. 2010 Feb;49(2):130-3. doi: 10.1016/j.cyto.2009.11.009. Epub 2009 Dec 8.
We investigated whether IFN-gamma gene +874(A/T) polymorphism influences intracellular interferon gamma expression in T-cell subsets of normal healthy subjects (NHS) and pulmonary tuberculosis patients (PTB). Peripheral blood mononuclear cells were stimulated with live Mycobacterium tuberculosis (MTB) and the intracellular IFN-gamma expression was studied using flow cytometry. Genotyping of IFN-gamma gene +874(A/T) was done using allele specific polymerase chain reaction. Significantly increased IFN-gamma expressing CD3+CD4+ and CD3+CD8+ T cells were observed in NHS with AA genotype compared to TT genotype in unstimulated (p=0.0308 and p=0.0157) and MTB stimulated (p=0.0494 and p=0.0287) cultures and this difference was not observed in PTB patients. The present study suggests that the variant genotypes of IFN-gamma (+874) may be associated with altered expression of IFN-gamma at the intracellular level and play an immunoregulatory role at the site of M. tuberculosis infection.
我们研究了 IFN-γ 基因+874(A/T) 多态性是否影响正常健康受试者（NHS）和肺结核患者（PTB）的 T 细胞亚群中的细胞内干扰素 γ 表达。使用活结核分枝杆菌（MTB）刺激外周血单核细胞，并使用流式细胞术研究细胞内 IFN-γ 表达。使用等位基因特异性聚合酶链反应对 IFN-γ 基因+874(A/T)进行基因分型。与 TT 基因型相比，AA 基因型的 NHS 中未刺激（p=0.0308 和 p=0.0157）和 MTB 刺激（p=0.0494 和 p=0.0287）培养物中 CD3+CD4+和 CD3+CD8+T 细胞中表达的 IFN-γ 增加，而在 PTB 患者中未观察到这种差异。本研究表明，IFN-γ（+874）的变异基因型可能与细胞内 IFN-γ表达的改变有关，并在 MTB 感染部位发挥免疫调节作用。
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