Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA.
Int J Parasitol. 2010 Feb;40(2):157-61. doi: 10.1016/j.ijpara.2009.11.010. Epub 2009 Dec 11.
Infection of C3HeB/FeJ and C57BL/6 mice with Leishmania major stimulates a healing cell-mediated immune response, while Leishmania amazonensis infection leads to chronic disease. Here we show C3HeB/FeJ mice co-infected with both species of Leishmania heal, while co-infected C57BL/6 mice do not. Using an in vitro killing assay we determined B cells from infected C57BL/6 mice are ineffective in promoting parasite killing compared with B cells from infected C3HeB/FeJ mice. Furthermore, infected C57BL/6 mice produce less antigen-specific antibodies compared with infected C3HeB/FeJ mice. These findings suggest B cells play a required role in the cell-mediated immune response against L. amazonensis.
C3HeB/FeJ 小鼠和 C57BL/6 小鼠感染利什曼原虫后会引发治愈性的细胞免疫应答，而感染莱什曼原虫则会导致慢性疾病。在这里，我们发现同时感染这两种利什曼原虫的 C3HeB/FeJ 小鼠能够被治愈，而同时感染的 C57BL/6 小鼠则不能。通过体外杀伤试验，我们发现与感染 C3HeB/FeJ 小鼠的 B 细胞相比，感染 C57BL/6 小鼠的 B 细胞在促进寄生虫杀伤方面效果不佳。此外，与感染 C3HeB/FeJ 小鼠相比，感染 C57BL/6 小鼠产生的抗原特异性抗体较少。这些发现表明 B 细胞在针对莱什曼原虫的细胞免疫应答中发挥了必需的作用。
