Department of Biology, St Mary's College of Maryland, 18952 E Fisher Road, St Mary's City, Maryland 20686, USA.
J Clin Neurosci. 2010 Jan;17(1):103-6. doi: 10.1016/j.jocn.2009.08.003. Epub 2009 Dec 9.
Pharmaceutical agents known to be toxic to the human auditory system also impair sensory hair cells of teleosts, and this supports the use of fish models for the screening of such compounds. However, previous investigations have focused almost exclusively on anatomical changes after drug administration without assessing macro-level physiological effects. Using the goldfish (Carassius auratus), we demonstrate that the acquisition of auditory evoked potentials offers a rapid and non-invasive means for tracking ototoxin-induced shifts in auditory thresholds. Gentamicin (100mg/mL) was the agent of choice as it is an extensively-studied human ototoxin. Significant shifts (p<0.05) in hearing sensitivity were observed between 300 Hz and 600 Hz and these shifts depended on acoustic pressure, but not particle motion. This differential elevation of auditory thresholds may be caused by impairment of specific populations of auditory sensory hair cells.
已知对人体听觉系统有毒的药物制剂也会损害硬骨鱼类的感觉毛细胞，这支持了使用鱼类模型来筛选此类化合物。然而，以前的研究几乎完全集中在药物给药后的解剖结构变化上，而没有评估宏观水平的生理效应。使用金鱼（Carassius auratus），我们证明听觉诱发电位的获得提供了一种快速、非侵入性的方法来跟踪耳毒性物质引起的听觉阈值变化。庆大霉素（100mg/mL）是一种选择，因为它是一种被广泛研究的人类耳毒性物质。在 300Hz 和 600Hz 之间观察到听力敏感度的显著变化（p<0.05），这些变化取决于声压，而不取决于质点运动。听觉阈值的这种差异升高可能是由于特定种群的听觉感觉毛细胞受损所致。
