一种简单快速的细菌转化方法。

A simple and rapid method of bacterial transformation.

机构信息

Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.

出版信息

J Microbiol Methods. 2010 Feb;80(2):215-6. doi: 10.1016/j.mimet.2009.12.002.

DOI:10.1016/j.mimet.2009.12.002

PMID:20004690

Abstract

Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar.

摘要

最近，Naoto Yoshida 及其同事开发了一种使用纳米纤维接种 DNA 的独特细菌转化方法。我们已经验证了该原理，成功转化了大肠杆菌、肠炎沙门氏菌和鲍曼不动杆菌，并建立了一个用户友好的方案。将海泡石——一种廉价、纤维状但无害的矿物质——的缓冲悬浮液与细菌和转化 DNA 混合，并将混合物直接铺在选择性琼脂上。