Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Neuroscience. 2010 Mar 10;166(1):212-9. doi: 10.1016/j.neuroscience.2009.11.037. Epub 2009 Dec 14.
Physiological and anatomical investigations are commonly combined in experimental models. When studying the lower urinary tract (LUT), it is often of interest to perform both urodynamic studies and retrogradely labeled neurons innervating the peripheral target organs. However, it is not known whether the use of anatomical tracers for the labeling of, e.g. spinal cord neurons may interfere with the interpretation of the physiological studies on micturition reflexes. We performed cystometry and external urethral sphincter (EUS) electromyography (EMG) under urethane anesthesia in adult female rats at 5-7 days after injection of a 5% fluorogold (FG) solution or vehicle into the major pelvic ganglia (MPG) or the EUS. FG and vehicle injections into the MPG and EUS resulted in decreased voiding efficiency. MPG injections increased the duration of both bladder contractions and the inter-contractile intervals. EUS injections decreased EUS EMG bursting activity during voiding as well as increased both the duration of bladder contractions and the maximum intravesical pressure. In addition, the bladder weight and size were increased after either MPG or EUS injections in both the FG and vehicle groups. We conclude that the injection of anatomical tracers into the MPG and EUS may compromise the interpretation of subsequent urodynamic studies and suggest investigators to consider experimental designs, which allow for physiological assessments to precede the administration of anatomical tracers into the LUT.
生理和解剖学研究通常在实验模型中结合进行。在研究下尿路（LUT）时，通常有兴趣同时进行尿动力学研究和逆行标记支配周围靶器官的神经元。然而，目前尚不清楚使用解剖示踪剂标记，例如脊髓神经元是否会干扰对排尿反射的生理研究的解释。我们在成年雌性大鼠中进行了 5-7 天后的解剖学研究，将 5%荧光金（FG）溶液或载体注入主要盆神经节（MPG）或尿道外括约肌（EUS）中，然后在乌拉坦麻醉下进行膀胱测压和尿道外括约肌肌电图（EMG）记录。FG 和载体注入 MPG 和 EUS 导致排尿效率降低。MPG 注射增加了膀胱收缩的持续时间和收缩间期。EUS 注射减少了排尿过程中的尿道外括约肌 EMG 爆发活动，并增加了膀胱收缩的持续时间和最大膀胱内压。此外，在 FG 和载体组中，MPG 或 EUS 注射后，膀胱重量和大小均增加。我们得出结论，将解剖示踪剂注入 MPG 和 EUS 可能会影响随后的尿动力学研究的解释，并建议研究人员考虑实验设计，这些设计允许在将解剖示踪剂注入 LUT 之前进行生理评估。
