Barnard S M, Walt D R
Department of Chemistry, Tufts University, Medford, MA 02155.
Science. 1991 Feb 22;251(4996):927-9. doi: 10.1126/science.2000492.
A novel chemical sensor has been developed in which the polymer ethylene-vinyl acetate is used as a controlled-release system to deliver reagents to the sensing region of an optical fiber for a homogeneous competitive immunoassay based on fluorescence energy transfer. A competition reaction is used to enable continuous measurements of the solution antigen concentration. More generally, the technique allows irreversible indicating chemistries to be used in the construction of chemical sensors that can measure continuously for long periods. Although the sensor configuration has not been optimized in all respects, data are presented for a model system in which a fluorescein-labeled antibody and Texas Red-labeled immunoglobulin G (IgG) are used.
一种新型化学传感器已被开发出来，其中聚合物乙烯-醋酸乙烯酯被用作控释系统，将试剂输送到光纤的传感区域，用于基于荧光能量转移的均相竞争免疫分析。采用竞争反应来实现对溶液中抗原浓度的连续测量。更一般地说，该技术允许在构建能够长期连续测量的化学传感器时使用不可逆指示化学方法。尽管传感器配置在所有方面尚未优化，但给出了一个模型系统的数据，其中使用了荧光素标记的抗体和德克萨斯红标记的免疫球蛋白G（IgG）。
