State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, China.
Colloids Surf B Biointerfaces. 2010 Mar 1;76(1):381-5. doi: 10.1016/j.colsurfb.2009.11.013. Epub 2009 Nov 20.
Cell patterning on substrates has played a significant role in the study of basic biology, cell-based biosensor and tissue engineering. In this report, a cell pattern was prepared on poly(dimethylsiloxane) (PDMS) substrate by vacuum ultraviolet (VUV) lithography. After immobilizing allyl-polyethylene glycol (APEG) onto PDMS, a chemical heterogeneous patterned surface was fabricated by VUV (Xe(2) excimer: 172nm) lithography with copper mesh as a photomask. The UV exposed domains can promote L929 cell adhesion and growth. However, non-exposed regions resist cell attachment because of the repelling property of PEG. Therefore, cell pattern could be achieved without pre-adsorption of cell adhesive species before cell culture.
基底的细胞图案化在基础生物学、基于细胞的生物传感器和组织工程学的研究中发挥了重要作用。在本报告中，通过真空紫外（VUV）光刻在聚二甲基硅氧烷（PDMS）基底上制备了细胞图案。在将烯丙基聚乙二醇（APEG）固定到 PDMS 上之后，通过 VUV（Xe(2)准分子：172nm）光刻并用铜网作为掩模来制造化学异质图案化表面。UV 曝光区域可以促进 L929 细胞的黏附和生长。然而，由于 PEG 的排斥特性，非曝光区域抵抗细胞附着。因此，在细胞培养之前无需预先吸附细胞黏附物质即可实现细胞图案化。
