鉴定和分析登革热 1-4 型病毒感染的细胞培养物和组织中的小亚基因组 RNA。

Identification and characterization of small sub-genomic RNAs in dengue 1-4 virus-infected cell cultures and tissues.

机构信息

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.

出版信息

Biochem Biophys Res Commun. 2010 Jan 1;391(1):1099-103. doi: 10.1016/j.bbrc.2009.12.030. Epub 2009 Dec 11.

DOI:10.1016/j.bbrc.2009.12.030

PMID:20005199

Abstract

Dengue virus (DV) contains a single-stranded, positive-sense RNA genome, and the 3' non-coding regions (NCRs) have been demonstrated to play crucial roles in viral RNA replication and translation initiation. In this study, we report the presence of a species of small sub-genomic RNA (sgRNA) derived from the 3' NCR in DV-infected cell cultures and animal tissues. Further experiments demonstrate that these sgRNAs range from 390 to 430 nt in length and that they accumulate together with viral genomic RNA during replication. Sequence analysis and secondary structure prediction indicate that the stem-loop structures within the 3' NCR are responsible for the production of DV sgRNAs. Unexpectedly, a unique DV isolate produced three species of sgRNAs, compared to only one in the other isolates. Collectively, the structural and biochemical properties of DV sgRNAs warrant further study of their biological functions during the viral life cycle and pathogenesis.

摘要

登革病毒（DV）含有单链、正链 RNA 基因组，研究表明其 3'非编码区（NCR）在病毒 RNA 复制和翻译起始中发挥着关键作用。本研究报告了在 DV 感染的细胞培养物和动物组织中存在源自 3'NCR 的小亚基因组 RNA（sgRNA）。进一步的实验表明，这些 sgRNA 的长度为 390 至 430 个核苷酸，并且在复制过程中与病毒基因组 RNA 一起积累。序列分析和二级结构预测表明，3'NCR 内的茎环结构负责产生 DV sgRNA。出乎意料的是，与其他分离株相比，一种独特的 DV 分离株产生了三种 sgRNA。总的来说，DV sgRNA 的结构和生化特性证明了它们在病毒生命周期和发病机制中的生物学功能值得进一步研究。