Department of Molecular Genetics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Toxicol In Vitro. 2010 Apr;24(3):854-62. doi: 10.1016/j.tiv.2009.12.004. Epub 2009 Dec 11.
Urethane dimethacrylate (UDMA) is used in dental restorative materials in its polymeric form. However, the process of polymerization is usually incomplete and the monomers of UDMA can diffuse into the oral cavity and the pulp, reaching millimolar concentrations. In the present work we showed that UDMA at 0.1 and 1.0 mM decreased the viability of and induced DNA damage in lymphocytes in a concentration dependent manner, but it did not affect a plasmid DNA in vitro. UDMA at 1mM induced apoptosis in lymphocytes. The lymphocytes exposed to UDMA were able to repair their DNA within 60 min. Analysis with DNA repair enzymes Endo III and Fpg showed that UDMA induced mainly oxidative DNA lesions. Vitamin C and chitosan decreased genotoxic effect of UDMA. Our results show that monomers of UDMA may exert pronounced cyto- and genotoxic effects in human lymphocytes and chitosan can be considered as a protection against such effects.
异丁烯酸乙酯（UDMA）以聚合形式用于牙科修复材料。然而，聚合过程通常不完全，UDMA 的单体可以扩散到口腔和牙髓中，达到毫摩尔浓度。在本工作中，我们表明 UDMA 在 0.1 和 1.0mM 浓度下以浓度依赖的方式降低了淋巴细胞的活力并诱导了 DNA 损伤，但它不会影响体外的质粒 DNA。1mM 的 UDMA 诱导了淋巴细胞凋亡。暴露于 UDMA 的淋巴细胞能够在 60 分钟内修复其 DNA。用 DNA 修复酶 Endo III 和 Fpg 进行分析表明，UDMA 主要诱导氧化 DNA 损伤。维生素 C 和壳聚糖降低了 UDMA 的遗传毒性作用。我们的结果表明，UDMA 的单体可能对人淋巴细胞产生明显的细胞毒性和遗传毒性作用，壳聚糖可以被认为是对这种作用的一种保护。
