Xing X W, Hawthorne W J, Yi S, Simond D M, Dong Q, Ye B, Tong Q J, Ye Z, Wang W
Cell Transplantation and Gene Therapy Institute of Central South University, Changsha 410013, P. R. China.
Transplant Proc. 2009 Dec;41(10):4389-93. doi: 10.1016/j.transproceed.2009.09.051.
Porcine endogenous retrovirus (PERV) varies between pig breeds. Screening and analysis of PERV in putative pig breeds may provide basic parameters to evaluate the biological safety of xenotransplantation from pigs to humans. In this study, PERV was investigated among the conservation population of the Ningxiang pig. The result revealed that the genotype of PERV distribution was subtype A, 100%; subtype B, 100%; and subtype C, 100%. The env sequences of PERV-A and -B showed 11 clones detected by KpnI and MboI digestion, indicating that there existed multiple variants of PERV-A and -B in the Ningxiang pig. Reverse transcriptase polymerase chain reaction results showed that PERV had transcriptional activity in these individuals. In addition, PERV A/C recombinant was detected in most individuals of Ningxiang pig. Because PERV A/C recombinants increase the potential infectious risk, the breed may not be a proper donor for xenotransplantation.
猪内源性逆转录病毒（PERV）在不同猪种间存在差异。对假定猪种中的PERV进行筛选和分析可为评估从猪到人的异种移植的生物安全性提供基础参数。在本研究中，对宁乡猪的保种群进行了PERV调查。结果显示，PERV分布的基因型为A亚型，100%；B亚型，100%；C亚型，100%。PERV -A和 -B的env序列经KpnI和MboI酶切检测到11个克隆，表明宁乡猪中存在PERV -A和 -B的多种变体。逆转录聚合酶链反应结果显示PERV在这些个体中具有转录活性。此外，在宁乡猪的大多数个体中检测到了PERV A/C重组体。由于PERV A/C重组体增加了潜在的感染风险，该猪种可能不是异种移植的合适供体。
