Yang J, Li M, Zhang H, Hong L, Feng F, Pan F, Wang Y, Wang W
Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University of China, Xi'an 710032, Shaanxi Province, China.
Transplant Proc. 2009 Dec;41(10):4397-400. doi: 10.1016/j.transproceed.2009.09.085.
Lacking a long-term continuous intravenous infusion device for experimental animals is the biggest obstacle to constructing an allogenic small bowel transplantation rejection model.
To establish a rat heterotopic small bowel transplantation model with the use of a swivel device for intravenous transfusion.
The randomized block design of controlled observations included 90 inbred male F344/NCrl BR rats as donors and 90 LEW/Crl rats as recipients. On the basis of body weight, the rats were divided into three groups each with 30 pairs of rats. Group 1, the control group, underwent small bowel transplantation. Group 2, the transfusion group, received small bowel transplantation and continuous infusion of parenteral nutrient solution. Group 3, the tacrolimus (FK506) treatment group, received small bowel transplantation, continuous infusion of parenteral nutrient solution, and intravenous injection of FK506. The general status and survival time of transplanted rats were observed for 5 weeks.
The operative success rate was 100%. The survival rate was 83.3% (25/30) in the control group; and 96.7% (58/60) in groups 2 and 3 (P = .039). The mean survival time of the FK506 group was 22.1 +/- 13.4 days, which was significantly higher than that in the transfusion group (10.4 +/- 2.9 days, P = .023).
The simple, practical swivel intravenous infusion device provided treatment for more than 30 days, a useful tool for heterotopic segmental small bowel transplantation.
缺乏用于实验动物的长期持续静脉输注装置是构建同种异体小肠移植排斥模型的最大障碍。
使用旋转式静脉输血装置建立大鼠异位小肠移植模型。
采用随机区组设计的对照观察，90只近交系雄性F344/NCrl BR大鼠作为供体，90只LEW/Crl大鼠作为受体。根据体重将大鼠分为三组，每组30对。第1组为对照组，进行小肠移植；第2组为输血组，接受小肠移植并持续输注肠外营养液；第3组为他克莫司（FK506）治疗组，接受小肠移植、持续输注肠外营养液并静脉注射FK506。观察移植大鼠5周的一般状况和存活时间。
手术成功率为100%。对照组存活率为83.3%（25/30）；第2组和第3组为96.7%（58/60）（P = 0.039）。FK506组的平均存活时间为22.1±13.4天，显著高于输血组（10.4±2.9天，P = 0.023）。
简单实用的旋转式静脉输注装置可提供超过30天的治疗，是异位节段性小肠移植的有用工具。
