Kara B, Daglioglu K, Doran F, Akkiz H, Sandikci M, Kara I O
Department of Medical Oncology, Cukurova University, Adana, Turkey.
Transplant Proc. 2009 Dec;41(10):4401-4. doi: 10.1016/j.transproceed.2009.09.082.
It has been suggested that liver regeneration can occur either by differentiated adult hepatocytes which retain the capability for several rounds of replication or by hepatic progenitor cells, depending on the number of hepatocytes lost. We sought to study the differentiation potential of hepatocytes following partial hepatectomy (PH) in rats.
Using immunohistochemistry and confocal microscopy we studied the distribution of cytokeratin 7 (CK7), CK19, vimentin, desmin, CD34, and c-kit among adult rat liver hepatocytes after PH at various times just after hepatectomy and after 8, 16, 24, 36, 48, and 60 hour and 6 and 16 days.
Vimentin, c-kit, and desmin positivity were observed in regenerating hepatocytes in the early stages. Desmin and vimentin staining were also demonstrated in stellate cells. Staining enhancement in stellate cells progressed from day 3 to day 6. No liver sections were stained positive for CD34, CK19, or CK7.
After PH, mature hepatocytes revealed their potential to regain the markers that they do not express when they are quiescent. This result supported the plasticity and differentiation potential of adult hepatocytes during regeneration.
有研究表明，肝再生可通过保留多轮复制能力的分化成熟肝细胞或肝祖细胞发生，这取决于肝细胞损失的数量。我们旨在研究大鼠部分肝切除（PH）后肝细胞的分化潜能。
运用免疫组织化学和共聚焦显微镜技术，我们研究了在肝切除术后不同时间点（术后即刻、8、16、24、36、48和60小时以及6和16天）成年大鼠肝脏肝细胞中细胞角蛋白7（CK7）、CK19、波形蛋白、结蛋白、CD34和c-kit的分布情况。
在再生肝细胞的早期观察到波形蛋白、c-kit和结蛋白呈阳性。在星状细胞中也证实有结蛋白和波形蛋白染色。星状细胞中的染色增强从第3天持续到第6天。没有肝切片对CD34、CK19或CK7呈阳性染色。
部分肝切除后，成熟肝细胞显示出重新获得其静止时不表达的标志物的潜能。这一结果支持了成年肝细胞在再生过程中的可塑性和分化潜能。
