School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, United States.
J Neuroimmunol. 2010 Feb 26;219(1-2):17-24. doi: 10.1016/j.jneuroim.2009.11.006. Epub 2009 Dec 14.
We report here that an epitope (aa, 83-95) derived from Acanthamoeba castellanii (ACA) induces clinical signs of experimental autoimmune encephalomyelitis (EAE) in SJL/J mice reminiscent of the disease induced with myelin proteolipid protein (PLP) 139-151. By using IA(s)/tetramers, we demonstrate that both ACA 83-95 and PLP 139-151 generate antigen-specific cross-reactive CD4 T cells and the T cells secrete identical patterns of cytokines and induce EAE with a similar severity. These results may provide insights into the pathogenesis of multiple sclerosis and ACA-induced granulomatous encephalitis.
我们在此报告，源自棘阿米巴（Acanthamoeba castellanii）的表位（aa83-95）可在 SJL/J 小鼠中诱导类似于髓鞘蛋白脂质蛋白（PLP）139-151 诱导的实验性自身免疫性脑脊髓炎（EAE）的临床症状。通过使用 IA(s)/tetramers，我们证明 ACA83-95 和 PLP139-151 均可产生抗原特异性交叉反应性 CD4 T 细胞，这些 T 细胞分泌相同模式的细胞因子，并以相似的严重程度引发 EAE。这些结果可能为多发性硬化症和棘阿米巴诱导的肉芽肿性脑炎的发病机制提供新的认识。
