Köveker G B, Graham L M, Burkel W E, Sell R, Wakefield T W, Dietrich K, Stanley J C
Department of Surgery, University of Michigan, Ann Arbor.
Surgery. 1991 Mar;109(3 Pt 1):313-9.
The importance of blood and extracellular matrix precoating of expanded polytetrafluoroethylene (ePTFE) grafts on the effectiveness of endothelial cell (EC) seeding was assessed in a canine experimental model. Part I of the study documented ex vivo platelet deposition in 256 ePTFE grafts, 6 cm x 4 mm internal diameter, after implantation as femoral artery-femoral vein or carotid artery-jugular vein arteriovenous shunts. These conduits were precoated with blood, fibronectin, laminin, or collagen type IV with laminin, after which they were seeded with enzymatically derived and cultivated venous canine endothelium at a density of 30,000 to 40,000 EC/cm2 of graft surface. Luminal deposition of Indium 111-labeled platelets, expressed as 10(8) platelets/cm2, at 30 minutes (n = 176) and 24 hours (n = 80), respectively, was 2.29 and 0.30 for blood, 2.83 and 0.37 for fibronectin, 0.99 and 0.08 for laminin, and 0.98 and 0.11 for collagen type IV with laminin. Part II of the study documented in vivo luminal EC coverage at 14 days of 6 cm x 4 mm internal diameter ePTFE femoral or carotid arterial grafts (n = 8) prepared in the same manner as part I ex vivo shunt grafts. EC coverage with blood, fibronectin, laminin, and collagen type IV with laminin preparation was 42%, 49%, 44%, and 52%, respectively. The graft:carotid artery ratio of luminal 6-keto-PGF1 alpha release at 14 days with these same four preparations was 0.38, 0.31, 0.35, and 0.32, respectively. Precoatings of ePTFE prostheses with fibronectin, laminin, and collagen type IV are known to enhance the initial attachment of seeded EC. Fibronectin caused an insignificant increase in early platelet accumulation; laminin or laminin with collagen type IV preparations were associated with significantly less (p less than 0.005) deposition of platelets when compared to whole blood preparations. Most importantly, none of the four preparation techniques resulted in different in vivo rates of EC growth or luminal release of prostacyclin from conduits studied 14 days after implantation.
在犬类实验模型中评估了膨体聚四氟乙烯（ePTFE）移植物的血液和细胞外基质预涂层对内皮细胞（EC）接种效果的重要性。该研究的第一部分记录了256个内径为6 cm×4 mm的ePTFE移植物在植入后作为股动脉-股静脉或颈动脉-颈静脉动静脉分流时的体外血小板沉积情况。这些导管分别用血液、纤连蛋白、层粘连蛋白或含层粘连蛋白的IV型胶原进行预涂层，之后以每平方厘米移植物表面30000至40000个EC的密度接种经酶处理并培养的犬类静脉内皮细胞。铟111标记的血小板在管腔内的沉积，以每平方厘米10⁸个血小板表示，在30分钟（n = 176）和24小时（n = 80）时，血液组分别为2.29和0.30，纤连蛋白组分别为2.83和0.37，层粘连蛋白组分别为0.99和0.08，含层粘连蛋白的IV型胶原组分别为0.98和0.11。该研究的第二部分记录了与第一部分体外分流移植物制备方式相同的内径为6 cm×4 mm的ePTFE股动脉或颈动脉移植物在14天时的体内管腔内EC覆盖情况。血液、纤连蛋白、层粘连蛋白和含层粘连蛋白的IV型胶原预涂层的EC覆盖率分别为42%、49%、44%和52%。使用这四种相同预涂层的移植物在14天时管腔内6-酮-前列环素F1α释放与颈动脉的比率分别为0.38、0.31、0.35和0.32。已知用纤连蛋白、层粘连蛋白和IV型胶原对ePTFE假体进行预涂层可增强接种的EC的初始附着。纤连蛋白使早期血小板积累略有增加；与全血预涂层相比，层粘连蛋白或含IV型胶原的层粘连蛋白预涂层导致的血小板沉积显著减少（p < 0.005）。最重要的是，在植入14天后研究的四种制备技术中，没有一种导致导管内EC生长的体内速率或前列环素的管腔释放有所不同。
