棕褐色位点对黑腹果蝇与 D. melanogaster 杂交雌蝇色素沉着的影响很小。
Little effect of the tan locus on pigmentation in female hybrids between Drosophila santomea and D. melanogaster.
机构信息
Department of Ecology and Evolution, The University of Chicago, 1101 E. 57 Street, Chicago, IL 60637, USA.
出版信息
Cell. 2009 Dec 11;139(6):1180-8. doi: 10.1016/j.cell.2009.10.033.
Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly pigmented species, is based on mutations in the cis-regulatory region of tan, inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea males can produce viable hybrids when mated to D. melanogaster females enables us to use the armamentarium of genetic tools in the latter species to study the genetic basis of this interspecific difference in pigmentation. Hybridization tests using D. melanogaster deficiencies that include tan show no evidence that this locus is involved in the lighter pigmentation of D. santomea females; rather, the pigmentation difference appears to involve at least four other loci in the region. Earlier results implicating tan may have been based on a type of transgenic analysis that can give misleading results about the genes involved in an evolutionary change.
先前关于 Drosophila santomea 的研究表明，与其他深色色素物种相比，其腹部缺乏色素沉着是由于 tan 的顺式调控区发生突变，从而使该基因在 D. santomea 雌雄个体的腹部表达失活。我们发现 D. santomea 雄性个体与 D. melanogaster 雌性个体交配后能够产生有活力的杂交种，这使我们能够利用后者的遗传工具来研究色素沉着这种种间差异的遗传基础。使用包含 tan 的 D. melanogaster 缺陷进行的杂交测试没有证据表明该基因座参与了 D. santomea 雌性个体色素沉着的变浅；相反，色素沉着差异似乎至少涉及该区域的另外四个基因座。先前暗示 tan 的结果可能基于一种可以提供有关参与进化改变的基因的误导性结果的转基因分析。
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