Department of Pediatric Surgery, Hubei Women and Children's Hospital, Wuhan 430070, People's Republic of China.
J Pediatr Surg. 2009 Dec;44(12):2339-42. doi: 10.1016/j.jpedsurg.2009.07.061.
The study aimed to analyze the expression and significance of interstitial cells of Cajal (ICC) at the obstruction site of congenital pelviureteric junction obstruction (PUJO).
Specimens of the strictured segment of the PUJ were obtained from 24 patients who were diagnosed to have PUJO (without aberrant vessels and adhesive band compression of ureteral junction) intraoperatively. In the control group, PUJ specimens were taken from 21 patients who had Wilms' tumor. Pelviureteric junction tissues were confirmed to be free of tumor invasion by histology. Immunohistochemistry with c-kit antibody was performed to detect the expression of ICC in specimens of the 2 groups. Quantitative analysis was made using image analysis technique and statistical analysis was carried out.
Immunoreactivity to ICC was predominantly detected in the muscle layers of PUJ. The mean area of ICC expression in the PUJO group was 14.86 +/- 1.37 x 10(4)microm(2) , which was lower than that in the control group (16.80 +/- 1.68) x 10(4)microm(2) (P < .01). The mean density of ICC expression in the PUJO was 0.207 +/- 0.020, which was also lower than that in the control group (0.262 +/- 0.026) (P < .05).
A reduction of the number of ICC may play an important role in the etiology and pathogenesis of PUJO.
分析先天性肾盂输尿管连接部梗阻（PUJO）梗阻部位的 Cajal 间质细胞（ICC）的表达及其意义。
术中获得 24 例诊断为 PUJO（无异常血管和输尿管交界处粘连带压迫）患者的狭窄段 PUJ 标本。对照组 21 例为 Wilms 瘤患者，组织学证实肾盂输尿管交界处无肿瘤侵犯。采用 c-kit 抗体免疫组化法检测 2 组 ICC 的表达。采用图像分析技术进行定量分析，并进行统计学分析。
ICC 的免疫反应主要在 PUJ 的肌层中检测到。PUJO 组 ICC 表达的平均面积为 14.86 ± 1.37×10^4μm^2，低于对照组的 16.80 ± 1.68×10^4μm^2（P<.01）。PUJO 组 ICC 表达的平均密度为 0.207 ± 0.020，也低于对照组的 0.262 ± 0.026（P<.05）。
ICC 数量的减少可能在 PUJO 的病因和发病机制中起重要作用。
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