Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.
Talanta. 2010 Jan 15;80(3):1287-91. doi: 10.1016/j.talanta.2009.09.026.
Single strand DNA (ssDNA) was used to modify nanogold to obtain a nanogold-aptamer resonance scattering (RS) probe (NGssDNA) for Hg(2+), based on the formation of stable thymine-Hg(2+)-thymine (T-Hg(2+)-T) mismatches and aggregation of the released nanogold particles. After removing the aggregated particles by filtrate membrane, the excess NGssDNA in the filtration solution exhibit catalytic effect on the gold particle reaction between HAuCl(4) and ascorbic acid (AA) that appear as RS peak at 596nm. When Hg(2+) concentration increased, the RS intensity at 596nm decreased. The decreased intensity is linear to Hg(2+) concentration in the range of 0.00008-0.888ng/mL Hg(2+), with detection limit of 0.000034ng/mL. The nanogold-aptamer catalytic RS assay was applied to determination of Hg(2+) in water with satisfactory results.
单链 DNA（ssDNA）被用于修饰纳米金，得到用于 Hg(2+)的纳米金-适体共振散射（RS）探针（NGssDNA），这是基于形成稳定的胸腺嘧啶-Hg(2+)-胸腺嘧啶（T-Hg(2+)-T）错配和释放的纳米金颗粒的聚集。通过滤膜去除聚集的颗粒后，过滤溶液中过量的 NGssDNA 对 HAuCl(4) 和抗坏血酸（AA）之间的金颗粒反应表现出催化作用，在 596nm 处出现 RS 峰。随着 Hg(2+)浓度的增加，在 596nm 处的 RS 强度降低。该强度的降低与 0.00008-0.888ng/mL Hg(2+)范围内的 Hg(2+)浓度呈线性关系，检测限为 0.000034ng/mL。纳米金-适体催化 RS 测定法已成功应用于水样中 Hg(2+)的测定。
