Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Nei-Hu, Taipei 114, Taiwan.
Am J Emerg Med. 2010 Jan;28(1):37-43. doi: 10.1016/j.ajem.2008.09.026.
Part of platelet function involves aggregation and activation. Activation leads to platelet P selectin expression and platelet-leukocyte aggregation. Hypertonic saline inhibits platelet aggregation, although the effects of hypertonic saline on platelet activation are not known. We evaluated the effects of hypertonic saline on platelet activation as measured by platelet P selectin expression and platelet-leukocyte aggregation.
Blood samples from healthy volunteers (n = 6) were treated in vitro with various solutions including 23.5%, 7.5%, 3%, and 0.9% saline; Ringer's solution; 5% dextrose in water; and 10% hydroxyethyl starch. Blood was diluted with each type of solution to 2.5%, 5%, 10%, 20%, and 30% (vol/vol) dilution. All blood samples were activated with adenosine diphosphate (20 micromol/L), stained with fluorochrome-conjugated antibodies, and analyzed by flow cytometry to measure platelet P selectin expression and platelet-leukocyte aggregation.
The 23.5% saline solution reduced P selectin expression at 20% and 30% dilutions and platelet-leukocyte aggregation at 10%, 20%, and 30% dilutions. The 7.5% solution saline had no effect on P selectin expression and significantly inhibited platelet-leukocyte aggregation only at 30% dilution. Other solutions had no effect on platelet P selectin expression or platelet-leukocyte aggregation.
Our data suggest that hypertonic saline does not affect platelet P selectin expression or platelet-leukocyte aggregation at therapeutic plasma concentrations but that an inhibitory effect occurs at supratherapeutic doses. Dilutions of other solutions caused the least disturbance of platelet activation.
血小板功能的一部分涉及聚集和激活。激活导致血小板 P 选择素表达和血小板-白细胞聚集。高渗盐水抑制血小板聚集，尽管高渗盐水对血小板激活的影响尚不清楚。我们评估了高渗盐水对血小板激活的影响，方法是测量血小板 P 选择素表达和血小板-白细胞聚集。
健康志愿者（n=6）的血液样本在体外用各种溶液处理，包括 23.5%、7.5%、3%和 0.9%盐水；林格氏液；5%葡萄糖水；和 10%羟乙基淀粉。血液用每种溶液稀释至 2.5%、5%、10%、20%和 30%（体积/体积）稀释度。所有血液样本均用二磷酸腺苷（20 微摩尔/L）激活，用荧光染料标记的抗体染色，并通过流式细胞术分析，以测量血小板 P 选择素表达和血小板-白细胞聚集。
23.5%盐水溶液在 20%和 30%稀释度下降低 P 选择素表达，在 10%、20%和 30%稀释度下降低血小板-白细胞聚集。7.5%盐水溶液对 P 选择素表达没有影响，但仅在 30%稀释度下显著抑制血小板-白细胞聚集。其他溶液对血小板 P 选择素表达或血小板-白细胞聚集没有影响。
我们的数据表明，高渗盐水在治疗性血浆浓度下不影响血小板 P 选择素表达或血小板-白细胞聚集，但在超治疗剂量下会产生抑制作用。其他溶液的稀释对血小板激活的干扰最小。
