结核分枝杆菌 ftsH 表达对压力和生存能力的响应。
Mycobacterium tuberculosis ftsH expression in response to stress and viability.
机构信息
Biochemistry Department, The University of Texas Health Science Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA.
出版信息
Tuberculosis (Edinb). 2009 Dec;89 Suppl 1(Suppl 1):S70-3. doi: 10.1016/S1472-9792(09)70016-2.
Abstract
FtsH is an essential membrane-bound protease that degrades integral membrane proteins as well as cytoplasmic proteins. We show that Mycobacterium tuberculosis (Mtb) ftsH expression levels are upregulated upon exposure to agents that produce reactive oxygen and nitrogen intermediates (ROI and RNI) and growth in macrophages. In partial support of this result is our observation that the Mtb merodiploid overexpressing ftsH shows increased resistance to ROI. ftsH transcript levels are downregulated during stationary phase and starvation. ftsH overexpression strain shows delayed growth and reduced viability in vitro and ex vivo. Finally, we show that the intracellular levels of FtsZ, an essential cell-division protein, are reduced in ftsH-overexpressing strain. Together, our results suggest that Mtb FtsH is a stress-response protein that promotes the pathogen's ability to deal with ROI stress and is possibly involved in the regulation of FtsZ levels.
摘要
FtsH 是一种必需的膜结合蛋白酶，可降解整合膜蛋白和细胞质蛋白。我们发现分枝杆菌（Mtb）ftsH 的表达水平在暴露于产生活性氧和氮中间体（ROI 和 RNI）的试剂以及在巨噬细胞中生长时会上调。部分支持这一结果的是我们的观察结果，即过度表达 ftsH 的 Mtb 部分二倍体显示出对 ROI 的更高抗性。ftsH 的转录水平在静止期和饥饿时下调。ftsH 过表达株在体外和体内显示生长延迟和活力降低。最后，我们发现，在过度表达 ftsH 的菌株中，一种必需的细胞分裂蛋白 FtsZ 的细胞内水平降低。总之，我们的结果表明，Mtb FtsH 是一种应激反应蛋白，可促进病原体应对 ROI 应激的能力，并且可能参与 FtsZ 水平的调节。
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