Hefei National Laboratory for Physical Sciences on Microscale, University of Science and Technology of China, No. 96, Jinzhai Road, Hefei 230026, China.
J Hazard Mater. 2010 Apr 15;176(1-3):901-5. doi: 10.1016/j.jhazmat.2009.11.121. Epub 2009 Nov 27.
A cloud point extraction (CPE) method for the preconcentration of ultra-trace aluminum in human albumin prior to its determination by graphite furnace atomic absorption spectrometry (GFAAS) had been developed in this paper. The CPE method was based on the complex of Al(III) with 1-(2-pyridylazo)-2-naphthol (PAN) and Triton X-114 was used as non-ionic surfactant. The main factors affecting cloud point extraction efficiency, such as pH of solution, concentration and kind of complexing agent, concentration of non-ionic surfactant, equilibration temperature and time, were investigated in detail. An enrichment factor of 34.8 was obtained for the preconcentration of Al(III) with 10 mL solution. Under the optimal conditions, the detection limit of Al(III) was 0.06 ng mL(-1). The relative standard deviation (n=7) of sample was 3.6%, values of recovery of aluminum were changed from 92.3% to 94.7% for three samples. This method is simple, accurate, sensitive and can be applied to the determination of ultra-trace aluminum in human albumin.
本文提出了一种用于石墨炉原子吸收光谱法（GFAAS）测定人白蛋白中超痕量铝之前的预浓缩方法，即浊点萃取（CPE）法。该方法基于 Al(III)与 1-(2-吡啶偶氮)-2-萘酚（PAN）的配合物，并用非离子表面活性剂 Triton X-114 作为非离子表面活性剂。详细研究了影响浊点萃取效率的主要因素，如溶液的 pH 值、配合剂的浓度和种类、非离子表面活性剂的浓度、平衡温度和时间等。用 10 mL 溶液对 Al(III)进行预浓缩，可获得 34.8 的富集因子。在最佳条件下，Al(III)的检测限为 0.06ng mL(-1)。7 个样品的相对标准偏差（n=7）为 3.6%，三个样品的铝回收率在 92.3%至 94.7%之间变化。该方法简单、准确、灵敏，可用于人白蛋白中超痕量铝的测定。
