预处理家禽粪便可提高苏云金芽孢杆菌生物农药的产量。

Pretreatment of poultry litter improves Bacillus thuringiensis-based biopesticides production.

机构信息

Middle East Technical University, Department of Biological Sciences, 06531 Ankara, Turkey.

出版信息

Bioresour Technol. 2010 Apr;101(7):2401-4. doi: 10.1016/j.biortech.2009.11.048. Epub 2009 Dec 14.

DOI:10.1016/j.biortech.2009.11.048

PMID:20006924

Abstract

Pretreated poultry litter was used in batch cultures for the production of Bacillus thuringiensis (Bt)-based biopesticide of lepidoptera- and diptera-specific Cry1 and Cry2, diptera-specific Cry4Ba and Cry11Aa and coleoptera-specific Cry3Aa toxins by Bt subsp. kurstaki 81, subsp. israelensis HD500 and subsp. tenebrionis 3203, respectively. Bt kurstaki 81 showed improved growth and produced more toxin in this medium as compared to other subspecies. Base and acid hydrolysis were tested as the methods of substrate pretreatment. The use of poultry litter pretreated with 2N HCl yielded 94% more bioinsecticidal protein than 2N NaOH-pretreated poultry litter when Bt kurstaki 81 was cultured. With appropriate pretreatment, poultry litter demonstrated potential as a valuable raw material for a low-cost complex medium to produce Bt-based biopesticides.

摘要

预处理禽粪被用于生产鳞翅目和双翅目特异性 Cry1 和 Cry2、双翅目特异性 Cry4Ba 和 Cry11Aa 以及鞘翅目特异性 Cry3Aa 毒素的苏云金芽孢杆菌（Bt）基于生物农药的批量培养，分别由 Bt 亚种 kurstaki 81、亚种 israelensis HD500 和亚种 tenebrionis 3203 产生。与其他亚种相比，Bt kurstaki 81 在该培养基中表现出更好的生长和产生更多的毒素。对碱和酸水解进行了作为底物预处理的方法的测试。当使用 2N HCl 预处理禽粪时，与使用 2N NaOH 预处理禽粪相比，Bt kurstaki 81 的培养物产生的生物杀虫蛋白多 94%。通过适当的预处理，禽粪显示出作为生产基于 Bt 的生物农药的低成本复杂培养基的有价值原料的潜力。