Great Lakes Forestry Centre, Sault Ste Marie, ON P6A 2E5, Canada.
J Gen Virol. 2010 Apr;91(Pt 4):915-8. doi: 10.1099/vir.0.017145-0. Epub 2009 Dec 9.
The baculovirus occlusion-derived virion (ODV) is required to spread virus infection among insect hosts via the per os route. The Autographa californica multicapsid nucleopolyhedrovirus P74 protein is an ODV envelope protein that is essential for ODVs to be infectious. P74 is anchored in the ODV envelope by a C-terminal transmembrane anchor domain and is N-terminally exposed on the ODV surface. In the present study, a series of N-terminal and C-terminal truncation mutants of P74 were evaluated for their ability to rescue per os infectivity of the P74-null virus, AcLP4. It was discovered that a P74 truncation mutant lacking the C-terminal transmembrane anchor domain of P74 was able to rescue per os infection. This result shows that a soluble form of P74 retains per os infectivity factor function and suggests that P74 may be complexed with other proteins in the ODV envelope.
杆状病毒出芽型病毒（ODV）是通过经口途径在昆虫宿主间传播病毒感染所必需的。美洲棉铃虫多角体核型多角体病毒 P74 蛋白是一种 ODV 囊膜蛋白，对 ODV 的感染性至关重要。P74 通过 C 端跨膜锚定结构域锚定在 ODV 囊膜上，并在 ODV 表面的 N 端暴露。在本研究中，评估了一系列 P74 的 N 端和 C 端截短突变体，以确定它们是否能够拯救 P74 缺失病毒 AcLP4 的经口感染能力。结果发现，缺失 P74 的 C 端跨膜锚定结构域的 P74 截短突变体能够拯救经口感染。这一结果表明，可溶性形式的 P74 保留了经口感染因子的功能，并提示 P74 可能与 ODV 囊膜中的其他蛋白质形成复合物。
