Instituto de Biotecnología, CICVyA, INTA-Castelar, Hurlingham, Argentina.
Intervirology. 2012;55(3):247-51. doi: 10.1159/000324538. Epub 2011 May 17.
Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV's surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells.
杆状病毒出芽衍生病毒（BVs）和包埋病毒（ODVs）在形态和功能上是不同的。ODVs 因其高经口感染性而负责昆虫宿主的初次感染。相比之下，BVs 对肠内皮细胞的感染能力很差，但在昆虫组织中高效地传播感染。P74 是 ODVs 中最重要的蛋白之一，它参与了这种病毒表型与中肠内皮细胞的附着。我们评估了用两种 P74 变体对苜蓿银纹夜蛾多核多角体病毒的 BVs 进行假型化的可能性及其对其口服感染性的影响。两种重组 BVs 都含有 P74 并以与野生型病毒相似的方式复制。然而，P74 存在于 BV 的表面并不会增强这种表型的口服感染性，这表明 P74 存在于出芽病毒粒子的膜中会干扰它们感染中肠细胞的机制。
