一类具有平滑肌细胞分化潜能的小鼠骨髓细胞亚群——简报。
A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report.
机构信息
Centre for Research in Vascular Biology, BioSciences Institute, University College Cork, College Road, Cork City, Ireland.
出版信息
Arterioscler Thromb Vasc Biol. 2010 Mar;30(3):533-5. doi: 10.1161/ATVBAHA.109.200097. Epub 2009 Dec 10.
OBJECTIVE
To determine whether CX(3)CR1(+) bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation.
METHODS AND RESULTS
CX(3)CR1(+) and CX(3)CR1(-) cells were isolated from marrow of CX(3)CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX(3)CR1(+) bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC.
CONCLUSIONS
CX(3)CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.
目的
确定 CX(3)CR1(+) 骨髓细胞是否具有平滑肌细胞 (SMC) 分化的能力。
方法和结果
从 CX(3)CR1 转基因小鼠的骨髓中分离出 CX(3)CR1(+) 和 CX(3)CR1(-) 细胞，并在 SMC 分化培养基中培养。表型和功能分析表明，只有 CX(3)CR1(+) 骨髓细胞表现出与成熟 SMC 相似的集落细胞生长、SMC 特异性蛋白表达、钙信号和收缩反应。
结论
CX(3)CR1 标记了一个骨髓细胞群体，该群体富含具有体外分化为 SMC 样细胞能力的祖细胞。
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