Department of Oral and Maxillofacial Surgery, Cabueñes Hospital Gijón, Spain.
J Biomater Appl. 2010 Nov;25(4):367-81. doi: 10.1177/0885328209353643. Epub 2009 Dec 11.
The aim of this study was to evaluate the ectopic bone formation using a novel serum-derived albumin scaffold and cultured human mandibular osteoblasts in nude mice. Osteoblasts were cultured with 10% human serum and plated in a novel spongy noncalcified protein scaffold prepared with plasmatic albumin crossed with a glutaraldehyde type agent. Hematoxylin-eosin staining revealed a bone-like extracellular matrix and in vitro mineralization was confirmed by von Kossa staining. Histological and immunohistochemical evaluation showed progression of mineralization in vivo. These results suggest the clinical feasibility of alveolar cells and albumin scaffold as a good alternative for bone regeneration.
本研究旨在评估新型血清白蛋白支架和培养的人下颌骨成骨细胞在裸鼠体内的异位骨形成。成骨细胞在 10%人血清中培养，并种植在一种新型海绵状非钙化蛋白支架上，该支架由血浆白蛋白与戊二醛交联剂制备而成。苏木精-伊红染色显示出类骨质细胞外基质，von Kossa 染色证实了体外矿化。组织学和免疫组织化学评价显示体内矿化进展。这些结果表明，牙槽细胞和白蛋白支架作为骨再生的良好替代物具有临床可行性。
