小干扰核糖核酸对过氧化物酶体增殖物激活受体γ基因介导的鸭脂肪生成的干扰。
Adipogenesis in ducks interfered by small interfering ribonucleic acids of peroxisome proliferator-activated receptor gamma gene.
机构信息
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
出版信息
Poult Sci. 2010 Jan;89(1):88-95. doi: 10.3382/ps.2009-00289.
Peroxisome proliferator-activated receptor gamma (PPARgamma) participates in adipocyte differentiation and maintenance, including the promotion of lipid storage in mammals. In the present study, 3 duck PPARgamma small interfering RNA (siRNA) expression plasmids were constructed to investigate the effect of downregulating the expression of PPARgamma on adipogenesis and fat accumulation in ducks. The results indicate that the 3 siRNA specific for conserved regions of PPARgamma can effectively inhibit expression of PPARgamma. It was demonstrated that the expression of lipoprotein lipase and adipocyte fatty acid-binding protein in duck adipose tissue is repressed when the expression of PPARgamma is downregulated by siRNA. At the same time, the weight of abdominal fat at 21 and 35 d of age is decreased significantly (P < 0.05) compared with the control. However, the triglyceride levels in serum and muscle are not affected when the mRNA of PPARgamma is repressed. The current study indicates that the suppression of PPARgamma reduces abdominal fat deposition and regulates adipogenesis in ducks.
过氧化物酶体增殖物激活受体 γ（PPARγ）参与脂肪细胞分化和维持，包括促进哺乳动物的脂质储存。本研究构建了 3 个鸭过氧化物酶体增殖物激活受体 γ 小干扰 RNA（siRNA）表达质粒，以研究下调 PPARγ 表达对鸭脂肪生成和脂肪积累的影响。结果表明，针对 PPARγ 保守区域的 3 个 siRNA 可有效抑制 PPARγ 的表达。研究表明，当 siRNA 下调 PPARγ 的表达时，鸭脂肪组织中脂蛋白脂肪酶和脂肪细胞脂肪酸结合蛋白的表达受到抑制。同时，与对照组相比，21 和 35 日龄时腹脂重量显著降低（P<0.05）。然而，当 PPARγ 的 mRNA 受到抑制时，血清和肌肉中的甘油三酯水平不受影响。本研究表明，抑制 PPARγ 可减少腹部脂肪沉积并调节鸭的脂肪生成。
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