Biomedical Sciences Research Institute, School of Biomedical Sciences, University of Ulster, Coleraine, Co. Londonderry BT52 1SA, UK.
J Water Health. 2010 Mar;8(1):83-91. doi: 10.2166/wh.2009.204.
Control of waterborne gastrointestinal parasites represents a major concern to water industries worldwide. In developed countries, pathogens in drinking water supplies are normally removed by sand filtration followed by chemical disinfection. Cryptosporidium spp. are generally resistant to common disinfection techniques and alternative control strategies are being sought. In the current study, the photocatalytic inactivation of C. parvum oocysts was shown to occur in buffer solution (78.4% after 180 min) and surface water (73.7% after 180 min). Viability was assessed by dye exclusion, excystation, direct examination of oocysts and a novel gene expression assay based on lactate dehydrogenase 1 (LDH1) expression levels. Collectively, this confirmed the inactivation of oocysts and scanning electron microscopy (SEM) confirmed cleavage at the suture line of oocyst cell walls, revealing large numbers of empty (ghost) cells after exposure to photocatalytic treatment.
控制水生消化道寄生虫是全世界水产业的主要关注点。在发达国家，饮用水供应中的病原体通常通过砂滤去除，然后进行化学消毒。隐孢子虫通常对常见的消毒技术具有抗性，因此正在寻求替代的控制策略。在当前的研究中，已经证明在缓冲溶液（180 分钟后为 78.4%）和地表水（180 分钟后为 73.7%）中可以通过光催化灭活微小隐孢子虫卵囊。通过染料排除、出囊、卵囊的直接检查以及基于乳酸脱氢酶 1 (LDH1) 表达水平的新型基因表达测定来评估活力。总的来说，这证实了卵囊的失活，扫描电子显微镜 (SEM) 证实了卵囊细胞壁缝线处的裂解，在暴露于光催化处理后，大量的空（幽灵）细胞。
