Sosa Vanessa, Zunino Pablo
Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, CP11600, Montevideo, Uruguay.
J Infect Dev Ctries. 2009 Dec 15;3(10):762-70. doi: 10.3855/jidc.232.
Proteus mirabilis, an important uropathogen that can cause complicated urinary tract infections (UTI), has emerged as a therapeutic problem following mutations that compromise the use of antimicrobial drugs. Due to the serious effects associated with uropathogenic P. mirabilis and the problems related to the use of antibiotics, it is necessary to develop alternative strategies for its control. The objective of this study was to assess the effect of Ibicella lutea extract, a South American indigenous plant, on growth, virulence and biofilm production of uropathogenic P. mirabilis.
This study was based on the extract generation and the assessment of its effect on bacterial features related to virulence. These assays involved determination of antibacterial activity, swarming motility, Western blot to assess expression of fimbriae and flagella, biofilms formation, haemagglutination, haemolysis, and electron microscopy.
I. lutea extract had an effect on bacterial growth rate and bacterial morphology. It also affected P. mirabilis swarming differentiation, hemagglutination and biofilm formation on glass and polystyrene. These findings suggest that I. lutea may have a role as an agent for the control of P. mirabilis UTI.
奇异变形杆菌是一种重要的尿路致病菌，可引起复杂性尿路感染（UTI），在发生损害抗菌药物使用的突变后，已成为一个治疗难题。由于尿路致病性奇异变形杆菌会产生严重影响以及抗生素使用相关问题，有必要制定控制它的替代策略。本研究的目的是评估南美本土植物黄花刺茄提取物对尿路致病性奇异变形杆菌的生长、毒力和生物膜形成的影响。
本研究基于提取物的制备及其对与毒力相关的细菌特性的影响评估。这些试验包括抗菌活性测定、群集运动性、用于评估菌毛和鞭毛表达的蛋白质免疫印迹、生物膜形成、血凝、溶血和电子显微镜检查。
黄花刺茄提取物对细菌生长速率和细菌形态有影响。它还影响奇异变形杆菌的群集分化、血凝以及在玻璃和聚苯乙烯上的生物膜形成。这些发现表明黄花刺茄可能作为一种控制奇异变形杆菌所致尿路感染的药物发挥作用。
